| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,404,742 | G→A | 83.5% | intergenic (+429/‑549) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,404,742 | 0 | G | A | 83.5% | 143.4 / 22.2 | 62 | intergenic (+429/‑549) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base G (4/6); new base A (22/29); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01 | |||||||||||
TAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAT > W3110S.gb/3404672‑3404807 | tAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCg > 1:5331/1‑71 (MQ=255) gCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGt < 1:1630545/70‑1 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAAt < 1:199360/71‑1 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAAt < 1:2371181/71‑1 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAAt < 1:443887/71‑1 (MQ=255) tGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATg > 1:2584729/1‑71 (MQ=255) cTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgtc > 1:704199/1‑71 (MQ=255) gCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCt < 1:441231/70‑1 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTaa > 1:507594/1‑57 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTaa > 1:2802190/1‑57 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAAt > 1:1039916/1‑58 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt > 1:1588496/1‑60 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCtt < 1:311887/70‑1 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTg > 1:212014/1‑71 (MQ=255) caTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc > 1:2185242/1‑71 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATc > 1:449854/1‑64 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc > 1:1239119/1‑70 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc > 1:1864707/1‑70 (MQ=255) tATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGa > 1:2199876/1‑71 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGAt < 1:1716288/70‑1 (MQ=255) aGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:1195235/71‑1 (MQ=255) gACGATTCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCt < 1:689106/53‑1 (MQ=255) gACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCt < 1:973278/53‑1 (MQ=255) aCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTaa > 1:1663323/1‑44 (MQ=255) aCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgtcgtc < 1:2473665/51‑1 (MQ=255) cGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCttt > 1:463842/1‑56 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt > 1:265920/1‑71 (MQ=255) aTGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCtt < 1:659932/54‑1 (MQ=255) aTGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCtt < 1:30055/54‑1 (MQ=255) aTGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCtt < 1:2793637/54‑1 (MQ=255) tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgcg < 1:1688838/59‑1 (MQ=255) cAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATcc > 1:64052/1‑69 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTg < 1:1637231/49‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa < 1:2527485/71‑1 (MQ=255) gAAAATTGGGTAATCCCCAGCAACCACTGCGTAAt > 1:1097778/1‑35 (MQ=255) gAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTg < 1:1704397/48‑1 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTTTCGCGATCCTGGCATGCCTACat > 1:488941/1‑70 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat > 1:2733092/1‑70 (MQ=255) aaaaTTGGGTAATCACCAGCAACCACTGCGTAATGTCGTCTAt > 1:2259406/1‑43 (MQ=38) aaaaTTGGGTAATCACCAGCAACCACTGCGTAATGTCGTCTATcg > 1:2262597/1‑44 (MQ=38) aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:489854/71‑1 (MQ=255) aTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt < 1:1157058/71‑1 (MQ=255) tAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc < 1:146564/51‑1 (MQ=255) tAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc < 1:1008269/51‑1 (MQ=255) aaTCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGCTCCTGGCATccc > 1:944622/1‑55 (MQ=38) tCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtg > 1:887192/1‑71 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:1751698/71‑1 (MQ=255) cccAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc > 1:1112532/1‑37 (MQ=255) cAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc > 1:96545/1‑35 (MQ=255) cAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa < 1:1091763/71‑1 (MQ=255) cAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa < 1:1583074/71‑1 (MQ=255) cAGCAACCACCGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa < 1:1106830/71‑1 (MQ=255) aGCAACCTCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAg < 1:651100/71‑1 (MQ=255) aGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAg < 1:2047356/71‑1 (MQ=255) aGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAg < 1:1122396/71‑1 (MQ=255) cAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg < 1:1466295/59‑1 (MQ=255) cAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa < 1:1747526/68‑1 (MQ=255) cAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAt < 1:2716913/71‑1 (MQ=255) cAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAt < 1:2228022/71‑1 (MQ=255) cAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAt < 1:2199610/71‑1 (MQ=255) aCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAt < 1:642308/69‑1 (MQ=255) aCCGCTGCGTAATGACGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa > 1:2765175/1‑66 (MQ=255) aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatt < 1:1322194/50‑1 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:2392248/1‑62 (MQ=255) | TAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAT > W3110S.gb/3404672‑3404807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |