| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,488,502 | C→T | 15.2% | L47L (CTG→TTG) | yijO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,488,502 | 0 | C | T | 15.2% | 117.8 / 12.5 | 46 | L47L (CTG→TTG) | yijO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (19/20); new base T (4/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTG > W3110S.gb/3488440‑3488561 | gcagTAACGGCCCTGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGTAAGGt < 1:1130293/71‑1 (MQ=255) aCGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGttt > 1:2445586/1‑71 (MQ=255) ggCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgt > 1:712920/1‑71 (MQ=255) cccGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:156385/70‑1 (MQ=255) cGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGAt < 1:2146243/71‑1 (MQ=255) tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTttgtt < 1:1753940/65‑1 (MQ=255) tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTgg < 1:1307081/51‑1 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGa > 1:929737/1‑56 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGa > 1:27993/1‑56 (MQ=255) ccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:1864325/60‑1 (MQ=255) tCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGAt < 1:355453/61‑1 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCt > 1:168241/1‑41 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCt > 1:454630/1‑41 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:1627193/1‑70 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:1781480/1‑70 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:2275801/1‑70 (MQ=255) cTTGAGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:2656651/1‑70 (MQ=255) cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGc < 1:1766343/70‑1 (MQ=255) cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGc < 1:1404500/70‑1 (MQ=255) tATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGaa < 1:2546908/39‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:724552/71‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:563015/71‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGaa > 1:666432/1‑37 (MQ=255) aGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:81295/1‑69 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:432253/45‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:970698/45‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGc < 1:881057/62‑1 (MQ=255) tCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:2377833/44‑1 (MQ=255) tCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:474657/44‑1 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGa > 1:2203971/1‑36 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:1907119/1‑71 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:409877/1‑71 (MQ=255) gATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg > 1:840277/1‑40 (MQ=255) gATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg > 1:1634182/1‑40 (MQ=255) ttttCCACGGCTGGAAATTGTGCTGGAAGGTGAGtt < 1:2624726/36‑1 (MQ=255) ttttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGtt > 1:2415134/1‑67 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt > 1:1353662/1‑39 (MQ=255) ttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGgcgc > 1:690256/1‑49 (MQ=255) ttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGgcgc > 1:1876242/1‑49 (MQ=255) tCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc < 1:2057310/70‑1 (MQ=255) ggCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg < 1:1596892/71‑1 (MQ=255) gAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTg < 1:1934555/69‑1 (MQ=255) aTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:1344190/47‑1 (MQ=255) tgtgCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAAc > 1:1254694/1‑42 (MQ=255) gtgCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAg > 1:1251485/1‑46 (MQ=255) tgCTGTAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:2364204/43‑1 (MQ=255) | GCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTG > W3110S.gb/3488440‑3488561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |