| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,497,705 | T→G | 76.4% | R20R (CGT→CGG) | gldA → | glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,497,705 | 0 | T | G | 76.4% | 98.2 / 12.7 | 38 | R20R (CGT→CGG) | gldA | glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (20/9); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
GAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGG > W3110S.gb/3497638‑3497770 | gAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTg > 1:2253193/1‑71 (MQ=255) aGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTgg > 1:2682442/1‑71 (MQ=255) aGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTgg > 1:1501097/1‑71 (MQ=255) aGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTg > 1:1179938/1‑70 (MQ=255) aGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTg < 1:1658108/70‑1 (MQ=255) aaTTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCg < 1:445732/71‑1 (MQ=255) aaTTATGGACCGCATTATTCAATCACCGGGTAAATACATACAGGGCGCTGATGTGATTAATCGTCTGGGCg < 1:1697967/71‑1 (MQ=255) aTGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATa > 1:1381138/1‑71 (MQ=255) tGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAAt < 1:2352044/69‑1 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTg < 1:981087/71‑1 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTg < 1:1887743/71‑1 (MQ=255) ccGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCg < 1:2126163/62‑1 (MQ=255) attCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCt > 1:307477/1‑71 (MQ=255) ttCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGc > 1:1875081/1‑69 (MQ=255) aCCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa > 1:2174965/1‑49 (MQ=255) tAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt < 1:711325/71‑1 (MQ=255) tAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCTAAt < 1:1980318/44‑1 (MQ=38) tAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt < 1:798812/71‑1 (MQ=255) aaTACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt < 1:1013399/70‑1 (MQ=255) aaTACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt > 1:2297025/1‑69 (MQ=255) aTACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg < 1:1494248/38‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt > 1:398531/1‑71 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1757910/71‑1 (MQ=255) aCATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa > 1:1457434/1‑38 (MQ=255) aCATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGa > 1:1724982/1‑37 (MQ=255) catggCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTggtggg > 1:1699536/4‑71 (MQ=255) aGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTggtgggt < 1:391217/71‑1 (MQ=255) gggCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTggtgggt > 1:831186/1‑70 (MQ=255) gggCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTggtggg > 1:626971/1‑69 (MQ=255) gcTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACa > 1:1084986/1‑70 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGt > 1:1403352/1‑71 (MQ=255) gtgATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtgg > 1:450509/1‑55 (MQ=255) tgATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGtt > 1:947120/1‑71 (MQ=255) aTTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtg > 1:2532139/1‑51 (MQ=255) aTTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtg > 1:2528812/1‑51 (MQ=255) aaTCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTa > 1:1535111/1‑69 (MQ=255) aaTCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg > 1:2121848/1‑71 (MQ=255) tCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt < 1:1503438/42‑1 (MQ=255) | GAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGG > W3110S.gb/3497638‑3497770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |