Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 582,879 G→A 11.3% intergenic (+596/‑25) ylcE → / → appY hypothetical protein/DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb582,8790GA11.3% 179.6 / 11.1 62intergenic (+596/‑25)ylcE/appYhypothetical protein/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (25/30);  new base A (4/3);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TAAATGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCA  >  W3110S.gb/582814‑582942
                                                                 |                                                               
taAATGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGtttt                                                             >  1:2463731/1‑70 (MQ=255)
    tGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAAttt                                                        >  1:581232/1‑71 (MQ=255)
       acaTCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTAt                                                     <  1:577413/71‑1 (MQ=255)
         atcatcCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGa                                                   <  1:984443/71‑1 (MQ=255)
          tcatcCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTAt                                                     <  1:2713368/68‑1 (MQ=255)
          tcatcCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTAt                                                     <  1:1868693/68‑1 (MQ=255)
          tcatcCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGa                                                   <  1:1128750/70‑1 (MQ=255)
            atcCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAg                                                <  1:98284/71‑1 (MQ=255)
             tcCTCATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAAttt                                                        <  1:2496417/62‑1 (MQ=255)
               cTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTa                                                      >  1:38201/1‑62 (MQ=255)
               cTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTg                                             >  1:2403210/1‑71 (MQ=255)
                 gATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTATTTTAATTTTATGATAGGTg                                             >  1:1721659/1‑69 (MQ=255)
                          tgtgTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAgg                                               >  1:2280311/1‑58 (MQ=255)
                            tgtATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTAt                                <  1:964275/71‑1 (MQ=255)
                            tgtATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTAt                                <  1:479144/71‑1 (MQ=255)
                             gtATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTAt                                                     <  1:1924469/49‑1 (MQ=255)
                              tATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:1934795/59‑1 (MQ=255)
                              tATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:2564357/59‑1 (MQ=255)
                              tATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:1309828/59‑1 (MQ=255)
                              tATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTa                                 >  1:1509912/1‑68 (MQ=255)
                                  tAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAg                                         >  1:977858/1‑56 (MQ=255)
                                  tAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGt                              >  1:232873/1‑67 (MQ=255)
                                  tAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTg                           >  1:407707/1‑70 (MQ=255)
                                      tGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGa                                        >  1:2473099/1‑53 (MQ=255)
                                       ggTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAg                                                >  1:1266181/1‑44 (MQ=255)
                                        gttgttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:2592109/49‑1 (MQ=255)
                                        gttgttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:1323412/49‑1 (MQ=255)
                                        gttgttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCaa                                          <  1:2595389/49‑1 (MQ=255)
                                        gttgttATTTGACTACTATCAACTTATTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCg                      >  1:2367507/1‑69 (MQ=255)
                                         ttgttATTTGACTACTATCAACTTGTTTTAATTTTATGATa                                                 <  1:1832030/41‑1 (MQ=255)
                                         ttgttATTTGACTACTATCAACTTATTTTAATTTTATGATAGGTGCAAGATgg                                     <  1:1382472/53‑1 (MQ=255)
                                           gttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGa                                        >  1:684668/1‑48 (MQ=255)
                                           gttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGa                                        >  1:389941/1‑48 (MQ=255)
                                           gttATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGtt                 >  1:1691535/1‑71 (MQ=255)
                                             tATTTGACTACTATCAACTTGTTTTAATTTTATGa                                                   <  1:2450392/35‑1 (MQ=255)
                                              aTTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGtttt               >  1:2716850/1‑70 (MQ=255)
                                               tttGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTc              <  1:547133/70‑1 (MQ=255)
                                               tttGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATAATGTTTGCTCCGTAGTTTTc              <  1:914205/70‑1 (MQ=255)
                                                ttGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGtt                             >  1:2649573/1‑54 (MQ=255)
                                                ttGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGtt                             >  1:1835558/1‑54 (MQ=255)
                                                   actactATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATg                               <  1:375071/49‑1 (MQ=255)
                                                       ctaTCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCaa     >  1:749179/1‑71 (MQ=255)
                                                       ctaTCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCaa     >  1:2190694/1‑71 (MQ=255)
                                                       ctaTCAACTTATTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCaa     >  1:366510/1‑71 (MQ=255)
                                                       ctaTCAACTTATTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCa      >  1:1960456/1‑70 (MQ=255)
                                                        taTCAACTTGTTTTAATTTTATGATAGGTGCAAGATg                                      <  1:648833/37‑1 (MQ=255)
                                                        taTCAACTTGTTTTAATTTTATGATAGGTGCAAGATg                                      <  1:1550074/37‑1 (MQ=255)
                                                        taTCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTa                                 <  1:2633010/42‑1 (MQ=255)
                                                        taTCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTg                           <  1:817120/48‑1 (MQ=255)
                                                        taTCAACTTATTTTAATTTTATGATAGGTGCAAGATGGATTATg                               <  1:378392/44‑1 (MQ=255)
                                                        taTCAACTTATTTTAATTTTATGATAGGTGCAAGATGGATTATg                               <  1:381662/44‑1 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGa                                    >  1:2492391/1‑37 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGa                                    >  1:2024097/1‑37 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATg                               <  1:1733147/42‑1 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATc   <  1:347541/70‑1 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCa  <  1:2323647/71‑1 (MQ=255)
                                                          tCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCa  >  1:1203441/1‑71 (MQ=255)
                                                            aaCTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGtt                             >  1:401095/1‑42 (MQ=255)
                                                              cTTGTTTTAATTTTATGATAGGTTCAATATGGATTATGTTTGCTCCGTAGTTTTc              <  1:1574006/55‑1 (MQ=255)
                                                               ttGTTTTAATTTTATGATAGGTGCAAGATGGATTa                                 >  1:1485335/1‑35 (MQ=255)
                                                               ttGTTTTAATTTTATGATAGGTGCAAGATGGATTa                                 >  1:1228100/1‑35 (MQ=255)
                                                               ttGTTTTAATTTTATGATAGGTGCAAGATGGATTATGtt                             <  1:203647/39‑1 (MQ=255)
                                                                 |                                                               
TAAATGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCA  >  W3110S.gb/582814‑582942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: