| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,500,231 | C→T | 85.3% | A115T (GCG→ACG) | yijE ← | predicted permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,500,231 | 0 | C | T | 85.3% | 78.4 / 8.0 | 34 | A115T (GCG→ACG) | yijE | predicted permease |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (10/19); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01 | |||||||||||
GCCGAAAGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTCCCCGCACCTCCGCTG > W3110S.gb/3500166‑3500298 | gCCGAAAGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGa < 1:2102111/71‑1 (MQ=255) cGAAAGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGaa > 1:1472159/1‑70 (MQ=255) cGAAAGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGaa < 1:1716196/70‑1 (MQ=255) aaGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAAt < 1:2040382/70‑1 (MQ=255) tCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAg < 1:585935/71‑1 (MQ=255) tCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAg < 1:565879/71‑1 (MQ=255) cAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAg > 1:376567/1‑70 (MQ=255) gAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGaa < 1:253572/55‑1 (MQ=255) gAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGaa < 1:1980888/55‑1 (MQ=255) gAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACAACCCAGAAc > 1:1141889/1‑71 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACg > 1:1093627/1‑70 (MQ=255) tCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGaa > 1:95988/1‑67 (MQ=255) gcgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACgtgg > 1:584151/1‑46 (MQ=255) aaTATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGta < 1:260937/71‑1 (MQ=255) ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACgg < 1:239098/60‑1 (MQ=255) ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATg < 1:138727/64‑1 (MQ=255) ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATcac > 1:2159916/1‑48 (MQ=255) gCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt > 1:1154007/1‑70 (MQ=255) gCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt > 1:2078735/1‑70 (MQ=255) cACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAgg < 1:2486968/71‑1 (MQ=255) cACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAg < 1:2671895/70‑1 (MQ=255) gTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATcacca > 1:2045433/1‑42 (MQ=255) gCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGta > 1:2475888/1‑52 (MQ=255) gCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGta > 1:1326422/1‑52 (MQ=255) ttCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTc < 1:2266379/55‑1 (MQ=255) tCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTc < 1:2145964/54‑1 (MQ=255) tCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATc < 1:1221275/60‑1 (MQ=255) cACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTcccc > 1:771703/1‑71 (MQ=255) ccGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTCCCCGc < 1:1992425/71‑1 (MQ=255) acaacGTGGCGAAAATCACCACCCAGAACGGCATGGtat < 1:981251/39‑1 (MQ=255) acaacGTGGCGAAAATCACCACCCAGAACGGCATGGtat < 1:1815386/39‑1 (MQ=255) acaacGTGGCGAAAATCACCACCCAGAACGGCATGGtat < 1:2389046/39‑1 (MQ=255) acaacGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGcc < 1:2342863/53‑1 (MQ=255) aacGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTCCCCGCACCTCCGCt < 1:1991230/71‑1 (MQ=255) acGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTCCCCGCACCTCCGCTg < 1:2200631/71‑1 (MQ=255) | GCCGAAAGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCTTCCCCGCACCTCCGCTG > W3110S.gb/3500166‑3500298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |