| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,504,714 | G→A | 100% | F711F (TTC→TTT) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,504,714 | 0 | G | A | 97.2% | 98.0 / ‑3.8 | 36 | F711F (TTC→TTT) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/21); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
GCCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCGGT > W3110S.gb/3504647‑3504780 | gCCCCATTTCGCGGGCCGCTTCGAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAaaaaaa > 1:203946/1‑71 (MQ=255) cgaTTTCGCGGGCGGCTTCGAGCCGTTGCTCCATCTGCTCGTTCAGTTCTTCGCCATTTTCAAAAAAATgg < 1:348437/69‑1 (MQ=255) ccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg > 1:2223440/1‑71 (MQ=255) ccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg < 1:363898/71‑1 (MQ=255) aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:1250857/71‑1 (MQ=255) tttCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAg < 1:189141/62‑1 (MQ=255) cgGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGAtgct < 1:1292635/71‑1 (MQ=255) ccGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAaaaaaa > 1:1002767/1‑56 (MQ=255) cGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTgccg < 1:2650082/70‑1 (MQ=255) gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa > 1:2637328/1‑48 (MQ=255) gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa > 1:318720/1‑48 (MQ=255) aTCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCa < 1:1276839/59‑1 (MQ=255) tCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCAc > 1:601918/1‑70 (MQ=255) tCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCAc > 1:1818237/1‑70 (MQ=255) tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg > 1:2238793/1‑71 (MQ=255) gCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:1672415/37‑1 (MQ=255) cTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGc < 1:1472457/70‑1 (MQ=255) gttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGa < 1:2434704/69‑1 (MQ=255) gttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGa < 1:270647/69‑1 (MQ=255) gttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGAt < 1:1842027/70‑1 (MQ=255) ttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCcac < 1:2533912/69‑1 (MQ=255) caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACg > 1:604699/1‑70 (MQ=255) caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACg < 1:1418742/70‑1 (MQ=255) caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACg < 1:1637133/70‑1 (MQ=255) caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAACAGGCACCAGCGATTCCACACg < 1:1220681/70‑1 (MQ=255) aTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGt < 1:2758764/70‑1 (MQ=255) gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCaa > 1:2342568/1‑40 (MQ=255) ccATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGt < 1:2075931/66‑1 (MQ=255) cATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGa < 1:301074/71‑1 (MQ=255) ttttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca < 1:1774591/58‑1 (MQ=255) ttttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATc < 1:1618962/71‑1 (MQ=255) ttCAAAAAAATGGTCGGTGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTa > 1:621488/1‑62 (MQ=255) ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg > 1:31598/1‑48 (MQ=255) ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCcaca > 1:582766/1‑58 (MQ=255) ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATcc < 1:1228190/70‑1 (MQ=255) ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:2480319/1‑71 (MQ=255) ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:2412560/1‑71 (MQ=255) cAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCgg < 1:1469938/70‑1 (MQ=255) cAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCGGt > 1:849796/1‑71 (MQ=255) | GCCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCGGT > W3110S.gb/3504647‑3504780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |