| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,560,351 | G→A | 16.4% | P62L (CCC→CTC) | rbn ← | tRNA processing exoribonuclease BN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,560,351 | 0 | G | A | 16.4% | 136.8 / 18.0 | 55 | P62L (CCC→CTC) | rbn | tRNA processing exoribonuclease BN |
| Reads supporting (aligned to +/- strand): ref base G (30/16); new base A (4/5); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
AGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAG > W3110S.gb/3560293‑3560421 | aggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgcggcg > 1:2744496/1‑70 (MQ=255) gcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATgg < 1:2263244/57‑1 (MQ=255) cagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaa > 1:682476/1‑69 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAg > 1:2674891/1‑61 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg > 1:745864/1‑71 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:1096630/1‑70 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:1456290/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcgc > 1:2269431/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGaa < 1:2292525/64‑1 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGaa < 1:789279/64‑1 (MQ=255) gTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGa < 1:1358166/62‑1 (MQ=255) gTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCaa < 1:725551/71‑1 (MQ=255) ttGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCaaa < 1:2710399/71‑1 (MQ=255) ttGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCaaa < 1:2099988/71‑1 (MQ=255) tGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaa > 1:2513794/1‑69 (MQ=255) ggCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaaaa > 1:368831/1‑71 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:1634367/71‑1 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:2753517/71‑1 (MQ=255) aaaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:1706211/1‑71 (MQ=255) aaaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:2129523/1‑71 (MQ=255) aCGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCg > 1:1853007/1‑71 (MQ=255) aCGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCg > 1:356874/1‑71 (MQ=255) gTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:1705655/55‑1 (MQ=255) gTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg > 1:2280596/1‑70 (MQ=255) tAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCa > 1:1350157/1‑71 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAAcc > 1:1717166/1‑62 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:2090506/1‑70 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:2145394/1‑70 (MQ=255) gAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTaa > 1:285433/1‑70 (MQ=255) gAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt > 1:776476/1‑71 (MQ=255) aaTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt < 1:1681570/70‑1 (MQ=255) aTGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGc > 1:905995/1‑53 (MQ=255) tGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:1884592/46‑1 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:209107/63‑1 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:1703781/1‑71 (MQ=255) cTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:1267559/1‑71 (MQ=255) cTGACGTCGGAAAACATAGGAAAAGCGGCGAAAAGCGCAAAAACAACTGCAACCAGCGGCACt < 1:1738807/63‑1 (MQ=255) aCGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCaa > 1:2560804/1‑71 (MQ=255) aCGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCaa > 1:1104393/1‑71 (MQ=255) tCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGa < 1:2675560/71‑1 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:2494378/1‑38 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:1413701/71‑1 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:1529627/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:16184/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:23782/1‑71 (MQ=255) ggAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:2275268/71‑1 (MQ=255) gAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:474669/70‑1 (MQ=255) aaaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca > 1:192130/1‑71 (MQ=255) aaaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca < 1:224413/71‑1 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca > 1:2360864/1‑70 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca > 1:1318837/1‑70 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca > 1:793212/1‑70 (MQ=255) aaCATGGGAAAAGCGTCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:2355430/67‑1 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAgg > 1:2238602/1‑71 (MQ=255) tGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCa < 1:2029042/57‑1 (MQ=255) tGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCa < 1:1473685/57‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCaa < 1:2291234/70‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCaa < 1:1498023/70‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCaa < 1:1154352/70‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAg < 1:900264/71‑1 (MQ=255) | AGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAG > W3110S.gb/3560293‑3560421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |