| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,572,896 | C→T | 33.3% | R171* (CGA→TGA) | ompL → | predicted outer membrane porin L |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,572,896 | 0 | C | T | 33.3% | 85.7 / 63.3 | 69 | R171* (CGA→TGA) | ompL | predicted outer membrane porin L |
| Reads supporting (aligned to +/- strand): ref base C (25/21); new base T (12/11); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GGCACCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCTTCCGT > W3110S.gb/3572830‑3572961 | ggCACCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGt > 1:2198078/1‑71 (MQ=255)ggCACCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGt > 1:2276837/1‑71 (MQ=255)ggCACCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGt > 1:424244/1‑71 (MQ=255) ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAAt < 1:1710613/71‑1 (MQ=255) tACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGa > 1:1432658/1‑71 (MQ=255) aCTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGAc > 1:244589/1‑71 (MQ=255) ggAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACtt > 1:2266744/1‑70 (MQ=255) ggAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACtt > 1:2069598/1‑70 (MQ=255) aaTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTaa < 1:8086/71‑1 (MQ=255) aaTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTaa < 1:2333214/71‑1 (MQ=255) aaTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTaa < 1:2727525/71‑1 (MQ=255) aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTaa < 1:165647/70‑1 (MQ=255) aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTaa < 1:2294403/70‑1 (MQ=255) ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTg < 1:2636423/58‑1 (MQ=255) ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTg < 1:1759957/58‑1 (MQ=255) ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTaa > 1:2790972/1‑69 (MQ=255) ttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTTAGTGAATGACTTTAAtag > 1:2769465/1‑70 (MQ=255) ttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAAtag > 1:2222643/1‑70 (MQ=255) ttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAAtag > 1:1232445/1‑70 (MQ=255) ttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAAtag > 1:1220104/1‑70 (MQ=255) aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAAt < 1:1650878/63‑1 (MQ=255) aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAAt < 1:2660571/63‑1 (MQ=255) aCCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACgg > 1:1837621/1‑71 (MQ=255) gATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAta > 1:308363/1‑58 (MQ=255) gATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAta > 1:315322/1‑58 (MQ=255) aTAAATTTTCCTATACATTTGAGCCACATTACTTCATGc < 1:1451028/39‑1 (MQ=255) tAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAg < 1:2329422/71‑1 (MQ=255) aaaTTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGa > 1:346939/1‑67 (MQ=255) ttCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGatcat < 1:1486140/70‑1 (MQ=255) tCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGatcatc < 1:122253/70‑1 (MQ=255) cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAt < 1:1834313/46‑1 (MQ=255) cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAt < 1:651176/46‑1 (MQ=255) ataCATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTaa < 1:2158767/43‑1 (MQ=255) taCATTTGAGCCACATTACTTCATGCGAGTGAATg > 1:969644/1‑35 (MQ=255) taCATTTGAGCCACATTACTTCATGCGAGTGAATGACttt < 1:2537630/40‑1 (MQ=255) taCATTTGAGCCACATTACTTCATGCGAGTGAATGACttt < 1:1766776/40‑1 (MQ=255) cATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGa > 1:2508939/1‑70 (MQ=255) cATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGaa > 1:2512506/1‑55 (MQ=255) cATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGaa > 1:647663/1‑55 (MQ=255) cATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGaa > 1:1068097/1‑55 (MQ=255) cATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTggg > 1:662736/1‑69 (MQ=255) tttGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt < 1:915019/71‑1 (MQ=255) tttGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt < 1:2156227/71‑1 (MQ=255) tttGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt < 1:2622781/71‑1 (MQ=255) tttGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt < 1:2452448/71‑1 (MQ=255) tGAGCCACATTACTTCATGCGAGTGAATGACTTTAAtag > 1:2163592/1‑39 (MQ=255) tGAGCCACATTACTTCATGCGAGTGAATGACTTTAAtag > 1:2541594/1‑39 (MQ=255) tGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCa > 1:2106303/1‑71 (MQ=255) aGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt < 1:2282177/71‑1 (MQ=255) aGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt < 1:2758371/71‑1 (MQ=255) aGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt < 1:409342/71‑1 (MQ=255) acaTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGa < 1:1671361/44‑1 (MQ=255) acaTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAaca > 1:1995811/1‑71 (MQ=255) caTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGa < 1:739875/43‑1 (MQ=255) caTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGa < 1:2492159/59‑1 (MQ=255) caTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGa < 1:254534/59‑1 (MQ=255) aTTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGatcatc < 1:2763640/51‑1 (MQ=255) ttACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGatcatc < 1:1037074/50‑1 (MQ=255) ttACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCa > 1:1500343/1‑62 (MQ=255) ttACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAaca > 1:2019063/1‑68 (MQ=255) ttACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa > 1:1857767/1‑58 (MQ=255) ttACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt > 1:881149/1‑71 (MQ=255) tACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat > 1:1082833/1‑45 (MQ=255) aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTa > 1:1551566/1‑63 (MQ=255) aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCTTc > 1:1478578/1‑71 (MQ=255) aCTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGAtcatca > 1:92698/1‑49 (MQ=255) aCTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCTTc > 1:936149/1‑71 (MQ=255) ttCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt < 1:1177189/60‑1 (MQ=255) cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat > 1:1801337/1‑40 (MQ=255) cATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCTTCCGt > 1:1257475/1‑70 (MQ=255) cGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaaa > 1:1662836/1‑49 (MQ=255) cGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaaa > 1:2320102/1‑49 (MQ=255) cGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTaa > 1:960754/1‑56 (MQ=255) | GGCACCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCTTCCGT > W3110S.gb/3572830‑3572961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |