| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,582,634 | A→G | 100% | D411G (GAC→GGC) | glnG → | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,582,634 | 0 | A | G | 88.9% | 48.6 / ‑0.8 | 18 | D411G (GAC→GGC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (6/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTA > W3110S.gb/3582565‑3582701 | cAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGcaga > 1:645739/1‑70 (MQ=255) tGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGggcaggca > 1:1698332/1‑65 (MQ=255) tGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAgcgc > 1:2286185/1‑71 (MQ=255) tACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCg > 1:2086398/1‑71 (MQ=255) cTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGt > 1:964819/1‑71 (MQ=255) cTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGt > 1:366280/1‑71 (MQ=255) aaCCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTg < 1:2331193/71‑1 (MQ=255) cGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCa < 1:2415746/71‑1 (MQ=255) gCTTCTTGCGCAGTGGGCAGGCAGAGCTCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCag > 1:2758456/1‑71 (MQ=255) gCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGc > 1:1591047/1‑68 (MQ=255) tcttGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGc > 1:2261233/1‑71 (MQ=255) cttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCAAGAGc < 1:2757845/70‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTc < 1:226538/44‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTc < 1:674147/44‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTc < 1:1320899/44‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTc < 1:625244/44‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGaa < 1:639950/48‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGaa < 1:2360878/48‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGaa < 1:2770790/48‑1 (MQ=255) tGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGa < 1:1450627/70‑1 (MQ=255) ggcaggcaGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAg > 1:2285224/1‑47 (MQ=255) ggcaggcaGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGt > 1:906687/1‑71 (MQ=255) gacagaGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTa < 1:2059434/69‑1 (MQ=255) | CAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTA > W3110S.gb/3582565‑3582701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |