| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,587,844 | T→C | 68.2% | P624P (CCA→CCG) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,587,844 | 0 | T | C | 68.2% | 58.5 / 34.9 | 44 | P624P (CCA→CCG) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (3/11); new base C (14/16); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
AGCGGCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGGCGCGCCACCC > W3110S.gb/3587779‑3587907 | aGCGGCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa > 1:520185/1‑71 (MQ=255) cGGCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACg < 1:193437/71‑1 (MQ=255) gCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGa < 1:1421795/71‑1 (MQ=255) gCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGa < 1:114500/71‑1 (MQ=255) gCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGa < 1:2170662/71‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGa > 1:683873/1‑69 (MQ=255) gCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAg < 1:1349635/71‑1 (MQ=255) ttGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTc > 1:340237/1‑71 (MQ=255) tGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGt > 1:2373899/1‑69 (MQ=255) tGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTc > 1:942946/1‑70 (MQ=255) tGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTc > 1:2344059/1‑70 (MQ=255) tgtAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGcc < 1:2609674/71‑1 (MQ=255) tgtAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGcc < 1:919607/71‑1 (MQ=255) tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTcc < 1:704331/63‑1 (MQ=255) ggTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGt < 1:591778/70‑1 (MQ=255) ggTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGt < 1:2365508/70‑1 (MQ=255) tCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGttct < 1:1970447/71‑1 (MQ=255) cGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGttctt > 1:860053/1‑71 (MQ=255) aTTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGa < 1:1934938/50‑1 (MQ=255) tttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAg < 1:434826/52‑1 (MQ=255) tttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGa < 1:2096140/49‑1 (MQ=255) ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAg > 1:1176360/1‑71 (MQ=255) tCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAg > 1:1589482/1‑70 (MQ=255) tCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAg < 1:296899/70‑1 (MQ=255) tCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATa > 1:1066302/1‑49 (MQ=255) cAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGt < 1:2260121/70‑1 (MQ=255) gCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTAcc < 1:1973404/71‑1 (MQ=255) cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGt < 1:2713793/67‑1 (MQ=255) ttCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGa < 1:2416231/42‑1 (MQ=255) ttCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGa < 1:431636/42‑1 (MQ=255) tCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATa > 1:1059863/1‑43 (MQ=255) tCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATa > 1:901999/1‑43 (MQ=255) tCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATa > 1:2798258/1‑43 (MQ=255) cACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGtt < 1:2547103/71‑1 (MQ=255) aCTCCAGAATCACTTTTGGCAACGGATAGTCCATCGCCAGTTCTTCCa > 1:1654097/1‑48 (MQ=255) aCTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCa > 1:2175614/1‑48 (MQ=255) cTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGgc < 1:841334/70‑1 (MQ=255) tCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGt < 1:471895/39‑1 (MQ=255) ccAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGttcttc > 1:1228804/1‑43 (MQ=255) cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGgcgcgc < 1:728146/71‑1 (MQ=255) cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGgcgcgc < 1:1625034/71‑1 (MQ=255) gAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTAcc < 1:682944/47‑1 (MQ=255) gAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGGCGCGCCa < 1:2736430/71‑1 (MQ=255) tCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGGCGCGCCAccc > 1:2145487/1‑71 (MQ=255) | AGCGGCAGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACGGCGCGCCACCC > W3110S.gb/3587779‑3587907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |