Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,591,039 G→A 25.0% intergenic (+28/+13) yihG → / ← yihF predicted endonuclease/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,591,0390GA25.0% 41.5 / 11.0 24intergenic (+28/+13)yihG/yihFpredicted endonuclease/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (7/11);  new base A (3/3);  total (10/14)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCATCTC  >  W3110S.gb/3590973‑3591102
                                                                  |                                                               
cTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCg                                                               <  1:1140131/69‑1 (MQ=255)
          tcATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTAttt                                                    >  1:557409/1‑70 (MQ=255)
               tcatcaCAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTAttt                                                    >  1:1126637/1‑65 (MQ=255)
                  tcaCAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCgagtg                                                           <  1:1135054/55‑1 (MQ=255)
                          tCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTcc                                   <  1:978343/71‑1 (MQ=255)
                            aGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACgag                                                             >  1:1002199/1‑43 (MQ=255)
                            aGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACga                                                              >  1:1846326/1‑42 (MQ=255)
                             gAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGc                                                                <  1:1111583/39‑1 (MQ=255)
                             gAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGc                                                                <  1:460412/39‑1 (MQ=255)
                               aTAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCAc                              <  1:2496237/71‑1 (MQ=255)
                                taagtaagTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATg                                               <  1:808110/53‑1 (MQ=255)
                                taagtaagTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGta                                    <  1:1368628/64‑2 (MQ=255)
                                  agtaagtTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCg                                  >  1:2625070/1‑64 (MQ=255)
                                      agtTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACa                        >  1:1387617/1‑70 (MQ=255)
                                       gtTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATa                      <  1:2622429/71‑1 (MQ=255)
                                                gCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAAttcttc              <  1:2350113/70‑1 (MQ=255)
                                                   tctGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAAttcttcttc           >  1:1225355/1‑70 (MQ=255)
                                                     tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAAttc                 >  1:1999899/1‑62 (MQ=255)
                                                     tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCt        >  1:734186/1‑71 (MQ=255)
                                                      gAATGAGCAGAGGCGAGTGAGTATTTTAATGAAAAAACGTCCGGCACGAGACATaaa                    >  1:501181/1‑57 (MQ=255)
                                                       aaTGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCt        <  1:1433000/69‑1 (MQ=255)
                                                         tGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCAtc    <  1:1443035/71‑1 (MQ=255)
                                                           aGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCAtctc  <  1:2708024/71‑1 (MQ=255)
                                                           aGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCAtct   <  1:574093/70‑1 (MQ=255)
                                                                  |                                                               
CTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCATCTC  >  W3110S.gb/3590973‑3591102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: