| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,591,880 | C→T | 10.4% | M201I (ATG→ATA) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,591,880 | 0 | C | T | 10.4% | 194.5 / 10.8 | 67 | M201I (ATG→ATA) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (27/33); new base T (2/5); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACCGGAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCA > W3110S.gb/3591810‑3591949 | cAACCGGAAATTTCTTTTCTTATAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTc < 1:1774128/71‑1 (MQ=255) ggAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTgg > 1:616021/1‑71 (MQ=255) aaTTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGa > 1:757371/1‑71 (MQ=255) aTTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGAt < 1:1338173/71‑1 (MQ=255) aTTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGAt < 1:2540154/71‑1 (MQ=255) tttcttttctTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATa < 1:1829215/71‑1 (MQ=255) ttcttttcttCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCa > 1:2375024/1‑61 (MQ=255) ttcttttcttCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATaa > 1:29476/1‑71 (MQ=255) ttcttttcttCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCAGTTACTTTGTTCATTGGTGATaa > 1:1179380/1‑71 (MQ=255) ttcttcTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGc < 1:1072121/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACaa < 1:1958038/69‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAat < 1:612719/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAat < 1:1671155/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAat < 1:737990/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAat < 1:234640/70‑1 (MQ=255) gcgcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCt > 1:2567156/1‑71 (MQ=255) gcgcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCt > 1:130605/1‑71 (MQ=255) gagaATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCa > 1:516453/1‑39 (MQ=255) aaTCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATaa > 1:1067783/1‑46 (MQ=255) aaTCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTg > 1:2311968/1‑71 (MQ=255) tCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAatat > 1:919687/1‑58 (MQ=255) tCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGcc > 1:2418917/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAatat > 1:2114984/1‑57 (MQ=255) aaTGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAata > 1:2388593/1‑55 (MQ=255) aaTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACaa > 1:1598810/1‑53 (MQ=255) aTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc < 1:985537/71‑1 (MQ=255) aTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc < 1:596547/71‑1 (MQ=255) aTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc < 1:1474393/71‑1 (MQ=255) aTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc < 1:1662184/71‑1 (MQ=255) aTGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATATTCGACTTTGCCTTc < 1:2209825/71‑1 (MQ=255) tGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGAt < 1:1354331/38‑1 (MQ=255) tGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:105897/49‑1 (MQ=255) tGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:884425/49‑1 (MQ=255) tGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:644850/49‑1 (MQ=255) tGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc > 1:2808021/1‑70 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCa < 1:1778215/43‑1 (MQ=255) tAAAACTTTTTGCCGTTACTTTGTTCATTGGTGAt < 1:1850916/35‑1 (MQ=255) tAAAACTTTTTGCCGTTACTTTGTTCATTGGTGAt < 1:1227587/35‑1 (MQ=255) tAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:420994/46‑1 (MQ=255) tAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:2639478/46‑1 (MQ=255) aaaaCTTTTTGCCGTTACTTTGTTCATTGGTGATaa > 1:1194277/1‑36 (MQ=255) aaaaCTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGa > 1:1175457/1‑56 (MQ=255) aaaaCTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAatat > 1:468628/1‑71 (MQ=255) aCTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATc > 1:927207/1‑69 (MQ=255) cTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAg > 1:560817/1‑71 (MQ=255) tttttGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGAc < 1:284076/52‑1 (MQ=255) tttGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGt < 1:2688908/70‑1 (MQ=255) tttGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTCCCTTCAATATCCAGGt < 1:623154/70‑1 (MQ=255) ccGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGAc < 1:1835127/71‑1 (MQ=255) ttACTTTGTTTATTGGTGATAATTGCAGAACAATATc < 1:355587/37‑1 (MQ=255) ttACTTTGTTTATTGGTGATAATTGCAGAACAATATc < 1:2696655/37‑1 (MQ=255) ttACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGcc < 1:763472/49‑1 (MQ=255) ttACTTTGTTTATTGGGGATAATTGCAGAACAATATCTTCGACTTTGcc < 1:2222597/49‑1 (MQ=255) ttACTTTGTTCATTGGTGATAATTGCAGAACAATATc < 1:2551974/37‑1 (MQ=255) ttACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCa < 1:2240689/53‑1 (MQ=255) aCTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg < 1:589148/70‑1 (MQ=255) aCTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg < 1:1432486/70‑1 (MQ=255) cTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg < 1:214322/69‑1 (MQ=255) cTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGa < 1:1752927/70‑1 (MQ=255) tGTTCATTGGTGATAATTGCAGAACAATATCTTCGACt > 1:2236388/1‑38 (MQ=255) tGTTCATTGGTGATAATTGCAGAACAATATCTTCGACt > 1:1755799/1‑38 (MQ=255) tGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTc < 1:830577/46‑1 (MQ=255) tGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGAc > 1:975266/1‑62 (MQ=255) tCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAgg > 1:398983/1‑53 (MQ=255) tCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAgg > 1:1917265/1‑53 (MQ=255) tCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAgg > 1:279414/1‑53 (MQ=255) tCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACa > 1:2431071/1‑60 (MQ=255) tCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCa > 1:2258847/1‑71 (MQ=255) | CAACCGGAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCA > W3110S.gb/3591810‑3591949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |