| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,614,917 | G→A | 52.1% | G513G (GGC→GGT) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,614,917 | 0 | G | A | 52.1% | ‑1.6 / 69.8 | 48 | G513G (GGC→GGT) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (9/14); new base A (15/10); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGA > W3110S.gb/3614848‑3614985 | gATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGc > 1:416213/1‑71 (MQ=255) cAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc > 1:990997/1‑71 (MQ=255) cAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc > 1:1174636/1‑71 (MQ=255) ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:205823/71‑1 (MQ=255) ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:2110476/71‑1 (MQ=255) ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:1385617/71‑1 (MQ=255) ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:231798/71‑1 (MQ=255) gCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTaa > 1:1673029/1‑71 (MQ=255) aaCCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTaa > 1:1071810/1‑65 (MQ=255) ccGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAAt < 1:67486/71‑1 (MQ=255) cGGGCATCAGCCCCCATTCAGTTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAAt < 1:2686672/70‑1 (MQ=255) cATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTat < 1:1197271/48‑2 (MQ=255) tCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGt < 1:188502/55‑1 (MQ=255) tCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGcc < 1:1684641/61‑1 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTaa > 1:1088292/1‑44 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:1803137/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2725781/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2479351/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2796303/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:658176/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:68059/1‑70 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa < 1:992663/70‑1 (MQ=255) aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:1165927/1‑70 (MQ=255) ccATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAc > 1:1426278/1‑71 (MQ=255) cATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTa < 1:502254/43‑1 (MQ=255) cATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcg > 1:2471579/1‑71 (MQ=255) aTTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc < 1:2199701/71‑1 (MQ=255) ttCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc > 1:507224/1‑70 (MQ=255) ttCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc > 1:1134372/1‑70 (MQ=255) ttCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2404954/71‑1 (MQ=255) cAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1859387/1‑71 (MQ=255) cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1246856/1‑71 (MQ=255) cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:281987/1‑71 (MQ=255) cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1619663/1‑71 (MQ=255) cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:2404255/1‑71 (MQ=255) gTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaa < 1:1739042/57‑1 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGcc < 1:2031058/45‑1 (MQ=255) gCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaaa < 1:1380895/54‑1 (MQ=255) cTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTg < 1:2777513/71‑1 (MQ=255) caacaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTa > 1:543241/1‑71 (MQ=255) caacaaGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTa < 1:196981/71‑1 (MQ=255) aacaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2746013/55‑1 (MQ=255) acaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2778951/54‑1 (MQ=255) caaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACAt < 1:2412501/71‑1 (MQ=255) caaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACAt < 1:983359/71‑1 (MQ=255) aaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1616903/1‑54 (MQ=255) aaGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATg < 1:1100394/71‑1 (MQ=255) aTGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGAttt < 1:2366453/71‑1 (MQ=255) tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATaa > 1:2648179/1‑43 (MQ=255) tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATaa > 1:948471/1‑43 (MQ=255) tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTg < 1:768482/71‑1 (MQ=255) tgCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGa > 1:1101377/1‑70 (MQ=255) | GATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGA > W3110S.gb/3614848‑3614985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |