| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,626,383 | C→T | 12.1% | A33A (GCG→GCA) | yigL ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,626,383 | 0 | C | T | 12.1% | 90.3 / 4.3 | 33 | A33A (GCG→GCA) | yigL | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (13/16); new base T (2/2); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
CCAGATTATCGCGAATTTGCCCCACATCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCGGGAGAAAGTAA > W3110S.gb/3626313‑3626445 | ccAGATTATCGCGAATTTGCCCCACATCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCcgcgc > 1:900244/1‑71 (MQ=255) aTCGCGAATTTGCCCCACATCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTg < 1:1737968/70‑1 (MQ=255) cgcgAATTTGCCCCACATCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGt < 1:1433237/67‑1 (MQ=255) gAATTTGCCCCACATCAACGTGGTGACGACCGGTAGCAAACACAAAGTTGATGCCGCGCGCGGTTagcag < 1:2691428/70‑1 (MQ=255) tCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCttt > 1:1118026/1‑71 (MQ=255) tCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCttt > 1:455369/1‑71 (MQ=255) aaCGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGTGCGGTGAGCAGCTTCAGAGTTTCTTTgg > 1:1758437/1‑71 (MQ=255) aaCGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTg > 1:2332076/1‑70 (MQ=255) aaCGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTg > 1:2328994/1‑70 (MQ=255) aaCGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGCTTCTTTgg > 1:619765/1‑71 (MQ=255) gtggtgACGACCGGTCGCAATCACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCg > 1:1017707/1‑70 (MQ=255) gtggtgACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCtt > 1:1775825/1‑65 (MQ=255) tggttACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTa < 1:2048506/71‑1 (MQ=255) tggtgACGACCGGTCGCAAACACAAAGTTGATGCcgcgcgc < 1:73222/41‑1 (MQ=255) tggtgACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTa < 1:159788/71‑1 (MQ=255) tggtgACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTa < 1:2129793/71‑1 (MQ=255) gtgACGACCGGTCGCAAACACAAAGTTGATGCCGCGTGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTaa < 1:524934/70‑1 (MQ=255) tgACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGagca < 1:1725990/46‑1 (MQ=255) acgacCGGTCGCAAACAGAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAggg > 1:694187/1‑70 (MQ=255) acgacCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAgggg < 1:1891079/71‑1 (MQ=255) gacCGGTCGCAAACACAAAGTTGATGCCGCGTGCGGTGAGCAGCTTCag < 1:2595506/49‑1 (MQ=255) ccGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATaa > 1:697335/1‑71 (MQ=255) ggTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACg < 1:1786404/71‑1 (MQ=255) ggTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACg < 1:627818/71‑1 (MQ=255) ggTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGCATAACg < 1:2448842/71‑1 (MQ=255) gTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGt < 1:2759907/71‑1 (MQ=255) cGCAAACACAATGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTa < 1:2192031/57‑1 (MQ=255) gCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAg > 1:2061262/1‑58 (MQ=255) gCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATg > 1:1698250/1‑71 (MQ=255) acaAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCgg > 1:2499301/1‑70 (MQ=255) gTTGATGCCGCGTGCGGTGAGCAGCTTCAGAGTTTCTTTg > 1:1854259/1‑40 (MQ=255) gATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCGGGAGAAAGt < 1:550738/70‑1 (MQ=255) gATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCGGGAGAAAGt < 1:2725898/70‑1 (MQ=255) aTGCCGCGCGCGGTGAGCAGTTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCGGGAGAAAGTaa < 1:2393770/71‑1 (MQ=255) ccGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGc > 1:584572/1‑35 (MQ=255) gcgcgcgGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAg > 1:1359500/1‑38 (MQ=255) gcgcgcgGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAg > 1:1515045/1‑38 (MQ=255) gcgcgcgGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAg > 1:1985827/1‑38 (MQ=255) | CCAGATTATCGCGAATTTGCCCCACATCAACGTGGTGACGACCGGTCGCAAACACAAAGTTGATGCCGCGCGCGGTGAGCAGCTTCAGAGTTTCTTTGGCGTAAGGGGATAACGTATGGTCGGGAGAAAGTAA > W3110S.gb/3626313‑3626445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |