| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,671,661 | G→A | 15.9% | R204Q (CGG→CAG) | rhlB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,671,661 | 0 | G | A | 15.9% | 109.6 / 12.8 | 44 | R204Q (CGG→CAG) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (17/20); new base A (2/5); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
GGCTGTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGAA > W3110S.gb/3671592‑3671727 | ggctgTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCgg > 1:1388745/1‑71 (MQ=255) gctgTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCggg > 1:2351917/1‑71 (MQ=255) gctgTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCggg < 1:1640735/71‑1 (MQ=255) ttCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTa < 1:1270166/69‑1 (MQ=255) ttCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTa < 1:1339983/69‑1 (MQ=255) ccgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGt > 1:700711/1‑70 (MQ=255) ccgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGt > 1:668663/1‑70 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGTGTACGTGa < 1:2365284/71‑1 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGa < 1:1974246/71‑1 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTg < 1:422140/70‑1 (MQ=255) ccgccTGCAAACGAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCgg > 1:2360981/1‑54 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:38903/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:2366249/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:523019/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCAGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:579970/1‑68 (MQ=255) aCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGa < 1:2414010/52‑1 (MQ=255) aCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGa < 1:1102735/52‑1 (MQ=255) aGCGCCTCAACATGCTGTTCTCCTCCACGCTTTCGTACCGGGTACGTGAACTGTCGTTCGAGCAGATGAAc < 1:298752/71‑1 (MQ=255) aGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGaa > 1:132113/1‑70 (MQ=255) aGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:1259138/71‑1 (MQ=255) gcgcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:1351502/70‑1 (MQ=255) gcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTTAACTGGCGTTCGAGCAGATGAACaa < 1:1654807/70‑1 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg < 1:810970/56‑1 (MQ=255) cAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACa > 1:2355303/1‑65 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATg > 1:503360/1‑67 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:2054964/1‑70 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:1358583/1‑70 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg < 1:374005/70‑1 (MQ=255) aCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa < 1:721239/71‑1 (MQ=255) cATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa < 1:1466644/70‑1 (MQ=255) cATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa < 1:1017903/70‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata < 1:675031/71‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata < 1:17160/71‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata < 1:1704255/71‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata > 1:753707/1‑71 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata < 1:608178/71‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTg < 1:2029209/37‑1 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGaa > 1:1917177/1‑39 (MQ=255) gCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAatata > 1:395238/1‑71 (MQ=255) gCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGaa > 1:1643231/1‑37 (MQ=255) ttCTCCGCCACGCTTTCGTACCGGGTACGTGATCTGGCGTTCGAGCAGATGAACAATGCCGAATATATTg < 1:2765686/70‑1 (MQ=255) ttCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTg < 1:1529742/70‑1 (MQ=255) tctcCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTg > 1:1709625/1‑69 (MQ=255) ctcCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAg > 1:129481/1‑71 (MQ=255) aCGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:2671507/45‑1 (MQ=255) aCCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAAc > 1:26592/1‑60 (MQ=255) aCCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAAc > 1:171604/1‑60 (MQ=255) aCCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaa < 1:2340186/70‑1 (MQ=255) | GGCTGTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGAA > W3110S.gb/3671592‑3671727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |