| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,701,075 | T→C | 13.5% | L107L (TTA→TTG) | rbsC ← | D‑ribose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,701,075 | 0 | T | C | 13.5% | 89.7 / 7.9 | 37 | L107L (TTA→TTG) | rbsC | D‑ribose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/16); new base C (3/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701010‑3701145 | gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAAcgc < 1:1522669/71‑1 (MQ=255) tAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCgag < 1:1194315/70‑1 (MQ=255) aaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGagag > 1:1719798/1‑70 (MQ=255) cGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcag > 1:250393/1‑71 (MQ=255) cGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcag > 1:2286268/1‑71 (MQ=255) tGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcagcagc < 1:1487618/71‑1 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:2583133/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:1570162/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:1478943/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAAAGCGAGAGCAGCAGCGa > 1:974086/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGagcagcagc < 1:2009368/69‑1 (MQ=255) aCGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa < 1:954684/70‑1 (MQ=255) gACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGc < 1:1467988/70‑1 (MQ=255) cTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAg > 1:1117926/1‑66 (MQ=255) cTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGccac > 1:1091159/1‑70 (MQ=255) tCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAgc > 1:1525262/1‑71 (MQ=255) tCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAg > 1:2097826/1‑70 (MQ=255) gCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAgcg < 1:1271315/70‑1 (MQ=255) aCAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGccac > 1:1579265/1‑62 (MQ=255) aCAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCa > 1:84146/1‑69 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCAtt < 1:1190772/70‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:1075786/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:1107441/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:2576524/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGa > 1:1042888/1‑67 (MQ=255) ttACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGAc < 1:583116/61‑1 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAAt > 1:784/1‑66 (MQ=255) aCCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcga > 1:2793398/1‑70 (MQ=255) cACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:2433890/1‑69 (MQ=255) cACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATa < 1:1312796/71‑1 (MQ=255) aaTTGCGGCACCCAACGCGAGAGCAGCAGCGACAGccac > 1:2476243/1‑39 (MQ=255) ttGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAAt < 1:2427339/55‑1 (MQ=255) tGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa > 1:1418579/1‑53 (MQ=255) cGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcagc < 1:1420314/70‑1 (MQ=255) cGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcagca < 1:216936/71‑1 (MQ=255) ccTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga < 1:562909/55‑1 (MQ=255) ccTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga < 1:2205697/55‑1 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGAGTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:430239/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGa > 1:956682/1‑57 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:569687/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:2692343/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:1358274/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgc > 1:1050286/1‑69 (MQ=255) | GCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701010‑3701145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |