| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,712,033 | G→A | 13.6% | M328I (ATG→ATA) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,712,033 | 0 | G | A | 13.6% | 119.0 / 9.7 | 44 | M328I (ATG→ATA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (4/2); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTC > W3110S.gb/3711963‑3712100 | aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:2731331/71‑1 (MQ=255)aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg > 1:2557779/1‑71 (MQ=255)aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:2168636/71‑1 (MQ=255) tGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg > 1:2142641/1‑70 (MQ=255) aaaTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAg > 1:844342/1‑71 (MQ=255) aTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAggg < 1:353741/71‑1 (MQ=255) tttATCCGAACGGTATCACCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAggg > 1:741205/1‑70 (MQ=255) cGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAg > 1:2673726/1‑58 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:261164/1‑66 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:2588299/1‑66 (MQ=255) caccaGCCTTCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCg < 1:562605/71‑1 (MQ=255) accaTCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:139612/68‑1 (MQ=255) caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:1118948/1‑71 (MQ=255) caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:293488/1‑71 (MQ=255) caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:2544943/1‑71 (MQ=255) caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:1785681/1‑71 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg > 1:1530619/1‑71 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:662479/71‑1 (MQ=255) tGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCg > 1:1071157/1‑71 (MQ=255) tGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCATCCg > 1:709130/1‑71 (MQ=255) gCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:1631593/37‑1 (MQ=255) ccGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCggg > 1:2630739/1‑71 (MQ=255) gATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:1493362/71‑1 (MQ=255) aTGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGcc < 1:1245105/71‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCAtt < 1:2719860/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:990882/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:234595/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:926438/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:2136671/71‑1 (MQ=255) gATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg > 1:1574332/1‑70 (MQ=255) tGCAAATCGTCCGCTCTTTGCAGGGGATGGAAAACGCGAAGAt < 1:1998100/43‑1 (MQ=255) tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:2621849/43‑1 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:175606/1‑70 (MQ=255) aaaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc < 1:1632698/71‑1 (MQ=255) aaTCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt < 1:332897/67‑1 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:2656770/44‑1 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:1581078/44‑1 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:1988397/44‑1 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:2609402/1‑71 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:258362/1‑71 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:2140957/1‑71 (MQ=255) cGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActtct > 1:1643034/1‑71 (MQ=255) gTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:1699367/1‑50 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:887471/70‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:317619/71‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa > 1:26479/1‑71 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:184125/43‑1 (MQ=255) tATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc < 1:589507/58‑1 (MQ=255) tATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTc > 1:1811092/1‑71 (MQ=255) | ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTC > W3110S.gb/3711963‑3712100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |