| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,714,423 | C→T | 100% | H61Y (CAC→TAC) | atpI → | ATP synthase, membrane‑bound accesory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,714,423 | 0 | C | T | 87.0% | 115.8 / 10.7 | 46 | H61Y (CAC→TAC) | atpI | ATP synthase, membrane‑bound accesory subunit |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base T (25/15); total (30/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT > W3110S.gb/3714357‑3714490 | tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:1175372/1‑69 (MQ=255)tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:1581638/1‑69 (MQ=255)tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:222658/1‑69 (MQ=255) cAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg < 1:2431433/71‑1 (MQ=255) aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa > 1:801969/1‑71 (MQ=255) aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa < 1:2242807/71‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAg > 1:987298/1‑63 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATac < 1:1218446/71‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTATATGATATTTGCCTGGCGTTACCAGGCGCATa > 1:2789880/1‑70 (MQ=255) cTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa < 1:2342588/61‑1 (MQ=255) tGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa < 1:818542/60‑1 (MQ=255) gCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:2757824/1‑48 (MQ=255) gCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:1133601/1‑48 (MQ=255) gCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa < 1:2188436/56‑1 (MQ=255) gCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAgg > 1:1166083/1‑71 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg < 1:85085/71‑1 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg < 1:266774/71‑1 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa > 1:1193387/1‑53 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCggg > 1:815700/1‑71 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa > 1:2138153/1‑51 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa > 1:2761270/1‑51 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa > 1:2064562/1‑51 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:120470/1‑71 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:1964936/1‑71 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:514743/1‑71 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:265014/1‑71 (MQ=255) tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc > 1:2335345/1‑40 (MQ=255) tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg > 1:61649/1‑71 (MQ=255) tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATATACCAGCGAAAGGCCGGGt < 1:486556/69‑1 (MQ=255) cTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg < 1:291207/70‑1 (MQ=255) ttttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCg < 1:2001457/55‑1 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCggg > 1:1884801/1‑55 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCggg > 1:2469523/1‑55 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg > 1:2319341/1‑54 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCg > 1:54149/1‑70 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCg > 1:445710/1‑70 (MQ=255) ttGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCg > 1:2143821/1‑70 (MQ=255) tGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGc < 1:1804745/70‑1 (MQ=255) tttATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg < 1:2272402/51‑1 (MQ=255) tATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:594563/1‑51 (MQ=255) tatTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCggg > 1:6040/1‑45 (MQ=255) atTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAg > 1:1379039/1‑71 (MQ=255) tttGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGc > 1:2299468/1‑71 (MQ=255) gCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCaa < 1:430841/69‑1 (MQ=255) gCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCaa < 1:170993/69‑1 (MQ=255) cGTTACCAGGCGCATACACCAGCGAAAGGCCAGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGt < 1:643292/71‑1 (MQ=255) | TCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT > W3110S.gb/3714357‑3714490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |