| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,718,376 | G→A | 10.2% | intergenic (+9/‑42) | atpA → / → atpG | F1 sector of membrane‑bound ATP synthase, alpha subunit/F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,718,376 | 0 | G | A | 10.2% | 110.8 / 4.0 | 39 | intergenic (+9/‑42) | atpA/atpG | F1 sector of membrane‑bound ATP synthase, alpha subunit/F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/16); new base A (2/2); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
AAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAGAGA > W3110S.gb/3718315‑3718436 | aaGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCtt < 1:2412845/71‑1 (MQ=255) cTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAgg > 1:584166/1‑71 (MQ=255) gAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCtt < 1:1677475/61‑1 (MQ=255) aaGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAg > 1:1145735/1‑61 (MQ=255) aaGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCg > 1:2614388/1‑70 (MQ=255) aTCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAgg < 1:1347674/70‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCtt < 1:2750588/53‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCa < 1:1186831/59‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCa < 1:728590/71‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTAGGGCa < 1:289540/59‑1 (MQ=255) cTCGATTCCTTCAAAGCACCCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAg > 1:1470283/1‑53 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAggg > 1:714305/1‑55 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAggg > 1:42065/1‑55 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAggg > 1:2177037/1‑55 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAg > 1:863673/1‑53 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAg > 1:742694/1‑53 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCAtt > 1:1638468/1‑71 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCAtt > 1:700287/1‑71 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGc > 1:2570628/1‑48 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTAGGGCAgg > 1:51012/1‑59 (MQ=255) tCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTa > 1:1771778/1‑46 (MQ=255) tCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCAt > 1:857156/1‑64 (MQ=255) ccTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTgaggag < 1:2321276/70‑1 (MQ=255) ccTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTgaggag > 1:369019/1‑70 (MQ=255) ttCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCtt < 1:439026/37‑1 (MQ=255) aaaGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCt < 1:490141/70‑1 (MQ=255) aaaGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCt < 1:653522/70‑1 (MQ=255) aaaGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCt < 1:2332936/70‑1 (MQ=255) aaGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTg < 1:179855/59‑1 (MQ=255) aaGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCt < 1:102834/69‑1 (MQ=255) ccAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCgg > 1:671546/1‑71 (MQ=255) ccTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTg < 1:1743106/47‑1 (MQ=255) ccTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCa > 1:2075433/1‑59 (MQ=255) ccTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCa > 1:862556/1‑59 (MQ=255) ccTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCa > 1:132459/1‑70 (MQ=255) ccTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCa > 1:1383370/1‑70 (MQ=255) gTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCaa < 1:2087496/67‑1 (MQ=255) gTAACGTCTGGCAGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAaga < 1:202634/71‑1 (MQ=255) aCGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAgaga < 1:2214202/70‑1 (MQ=255) | AAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAGAGA > W3110S.gb/3718315‑3718436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |