| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 686,526 | A→G | 66.7% | I189T (ATC→ACC) | gltJ ← | glutamate and aspartate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 686,526 | 0 | A | G | 66.7% | 17.9 / 11.7 | 18 | I189T (ATC→ACC) | gltJ | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (6/6); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAT > W3110S.gb/686457‑686590 | aaCGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGa > 1:1157650/1‑70 (MQ=255)aaCGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGa > 1:2613747/1‑70 (MQ=255) tAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAg < 1:1483412/54‑1 (MQ=255) aTCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGa > 1:2651243/1‑56 (MQ=255) gAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCa < 1:1364421/71‑1 (MQ=255) gAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCAcc < 1:572413/70‑1 (MQ=255) gCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAgg < 1:2495378/71‑1 (MQ=255) tACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat < 1:1528000/70‑1 (MQ=255) cccGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatca < 1:2254461/70‑1 (MQ=255) tatCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTc < 1:581098/41‑1 (MQ=255) tatCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTc < 1:1454299/41‑1 (MQ=255) tCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:729345/1‑71 (MQ=255) tCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:1047529/1‑71 (MQ=255) tCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg < 1:1786205/71‑1 (MQ=255) tCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:1443648/1‑71 (MQ=255) tCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:1299946/1‑71 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:462440/1‑71 (MQ=255) caccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGa > 1:2415404/1‑71 (MQ=255) aGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGa > 1:223919/1‑70 (MQ=255) aGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:1274598/71‑1 (MQ=255) | AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAT > W3110S.gb/686457‑686590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |