| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,767,300 | A→G | 55.6% | I166V (ATA→GTA) | dgoA → | 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,767,300 | 0 | A | G | 55.6% | 2.4 / 19.5 | 18 | I166V (ATA→GTA) | dgoA | 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (4/6); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
TTGCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAATCCGTAG > W3110S.gb/3767237‑3767363 | ttGCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACg < 1:1809372/70‑1 (MQ=255)ttGCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATa < 1:1036450/66‑1 (MQ=255) gCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAg > 1:1219021/1‑71 (MQ=255) gCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAg > 1:1605685/1‑71 (MQ=255) gCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAg > 1:1861808/1‑71 (MQ=255) tCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCa < 1:1522026/68‑1 (MQ=255) tCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCa < 1:2555282/68‑1 (MQ=255) gCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTtg > 1:1885340/1‑62 (MQ=255) gTCTTTGCCGTTGGCTGCGTGACGCCAGAAAACCTGGCGCAGTGGGTa < 1:2189321/48‑1 (MQ=38) tttGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCa > 1:1063474/1‑60 (MQ=255) ttGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTtgtg < 1:2698844/57‑1 (MQ=255) ggcggcGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCg < 1:1082377/70‑1 (MQ=255) tGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGc < 1:1932817/43‑1 (MQ=255) aCGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATcg > 1:2385715/1‑71 (MQ=255) ccTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAAt > 1:939496/1‑71 (MQ=255) ccTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCg < 1:416111/50‑1 (MQ=255) cTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATctct < 1:305736/55‑1 (MQ=255) gcAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAATCCGTag < 1:528248/71‑1 (MQ=255) | TTGCCATCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAATCCGTAG > W3110S.gb/3767237‑3767363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |