| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,777,889 | T→C | 51.4% | V359A (GTG→GCG) | glvC → | arbutin specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,777,889 | 0 | T | C | 51.4% | ‑1.0 / 51.7 | 37 | V359A (GTG→GCG) | glvC | arbutin specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (12/6); new base C (12/7); total (24/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATG > W3110S.gb/3777820‑3777947 | cGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTtggtgtggtg < 1:2791310/71‑1 (MQ=255) gCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGc < 1:377612/70‑1 (MQ=255) cTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCaa < 1:2329508/71‑1 (MQ=255) cTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCaa < 1:2155463/71‑1 (MQ=255) cTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCaa < 1:1458205/71‑1 (MQ=255) cTGTTTGCAGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCaa < 1:1212027/71‑1 (MQ=255) tttGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAAc < 1:1501892/69‑1 (MQ=255) tGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATgg > 1:1572034/1‑71 (MQ=255) acacGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAg > 1:569726/1‑71 (MQ=255) cacGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAgg > 1:248079/1‑71 (MQ=255) cacGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg > 1:787906/1‑70 (MQ=255) ggcggcCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTggg < 1:1734477/45‑1 (MQ=255) cTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCt < 1:129269/57‑1 (MQ=255) cAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTa > 1:2805249/1‑71 (MQ=255) aaTGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACa > 1:288246/1‑41 (MQ=255) aaTGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTAc > 1:1862018/1‑71 (MQ=255) aaTGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTAc > 1:2106959/1‑71 (MQ=255) aTGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACa < 1:1141843/40‑1 (MQ=255) tGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCt < 1:2120667/52‑1 (MQ=255) tGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGAtt < 1:2758937/56‑1 (MQ=255) tCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATggg > 1:1929056/1‑41 (MQ=255) tCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGAtt > 1:1540292/1‑54 (MQ=255) tCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGAtt > 1:1583151/1‑54 (MQ=255) cGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCa > 1:2734796/1‑70 (MQ=255) gTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAAc > 1:1675325/1‑69 (MQ=255) gTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGt > 1:644548/1‑51 (MQ=255) gTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGa > 1:1019738/1‑45 (MQ=255) tATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATcc > 1:15876/1‑70 (MQ=255) tATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTc > 1:2026327/1‑37 (MQ=255) tATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAAc > 1:1568064/1‑63 (MQ=255) tATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATccc > 1:43602/1‑71 (MQ=255) tATCTCTTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATccc > 1:1306599/1‑71 (MQ=255) aTCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGa > 1:1786578/1‑43 (MQ=255) tctcTTTGGTGTGGTGGGCAACATGGGCGGAGGTCt < 1:412151/36‑1 (MQ=255) tctcTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGa > 1:1668084/1‑71 (MQ=255) tctcTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGa > 1:1864140/1‑71 (MQ=255) tctTTGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATg > 1:514878/1‑71 (MQ=255) tttGGTGTGGCGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGAt < 1:2388684/68‑1 (MQ=255) | CGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATG > W3110S.gb/3777820‑3777947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |