Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 141.3 / NA 50pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTA  >  W3110S.gb/3824303‑3824434
                                                                 |                                                                  
gAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACgg                                                               >  1:794802/1‑71 (MQ=255)
     cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTaaa                                                                  >  1:838214/1‑63 (MQ=255)
     cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                          >  1:1538054/1‑71 (MQ=255)
            cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                   >  1:2184014/1‑71 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCATTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                                <  1:1100532/71‑1 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                                 <  1:188839/70‑1 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                                <  1:196637/71‑1 (MQ=255)
                aaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                                <  1:1160993/70‑1 (MQ=255)
                   ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg                                            >  1:2304483/1‑71 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           <  1:1520686/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           <  1:2576568/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           <  1:1455672/71‑1 (MQ=255)
                     aacccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           >  1:696521/4‑70 (MQ=255)
                         cGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                                 <  1:470172/60‑1 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                   >  1:2717838/1‑70 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                   >  1:213824/1‑70 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                   >  1:81868/1‑70 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                       <  1:2386144/49‑3 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                  <  1:1714086/70‑1 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                  >  1:2375018/1‑70 (MQ=255)
                               aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                         >  1:507086/1‑62 (MQ=255)
                                   gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                >  1:1430861/1‑67 (MQ=255)
                                   gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGa                                 >  1:494218/1‑66 (MQ=255)
                                   gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAct                            >  1:1585396/1‑71 (MQ=255)
                                    aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                >  1:1795584/1‑66 (MQ=255)
                                    aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                <  1:1600943/66‑1 (MQ=255)
                                       gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActcag                         >  1:671196/1‑68 (MQ=255)
                                       gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                        >  1:1538357/1‑71 (MQ=255)
                                        tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                  <  1:1956780/60‑1 (MQ=255)
                                           ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                    <  1:2158226/71‑1 (MQ=255)
                                                gTTTCTGTCGTTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           <  1:2031462/43‑1 (MQ=16)
                                                gTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                           <  1:2558522/43‑1 (MQ=19)
                                                  ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                         >  1:2492081/1‑43 (MQ=19)
                                                  ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc               >  1:1915809/1‑69 (MQ=255)
                                                  ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc               >  1:1584950/1‑69 (MQ=255)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                         >  1:1762086/1‑42 (MQ=19)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                         >  1:1961018/1‑42 (MQ=19)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                         >  1:1962230/1‑42 (MQ=19)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                      >  1:2061158/1‑61 (MQ=255)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa            >  1:2554497/1‑71 (MQ=255)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa            >  1:356029/1‑71 (MQ=255)
                                                       tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACAt         >  1:324375/1‑70 (MQ=255)
                                                         ggtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa       >  1:2290447/4‑70 (MQ=255)
                                                          gtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                    <  1:2023681/54‑1 (MQ=255)
                                                          gtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                    <  1:2218138/54‑1 (MQ=255)
                                                           taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg    <  1:1129151/70‑1 (MQ=255)
                                                           taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg    <  1:2759167/70‑1 (MQ=255)
                                                            aTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga                  >  1:1376371/1‑56 (MQ=255)
                                                             ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa  <  1:2508332/71‑1 (MQ=255)
                                                             ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa  <  1:2071002/71‑1 (MQ=255)
                                                             ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa  <  1:1975538/71‑1 (MQ=255)
                                                                 |                                                                  
GAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTA  >  W3110S.gb/3824303‑3824434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: