| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 118,976 | G→A | 12.2% | G82S (GGT→AGT) | ampD → | N‑acetyl‑anhydromuranmyl‑L‑alanine amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 118,976 | 0 | G | A | 12.2% | 135.7 / 9.6 | 49 | G82S (GGT→AGT) | ampD | N‑acetyl‑anhydromuranmyl‑L‑alanine amidase |
| Reads supporting (aligned to +/- strand): ref base G (17/26); new base A (3/3); total (20/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GCACATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCA > W3110S.gb/118910‑119040 | gCACATCCTTTCTTTTCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGa < 1:385240/71‑1 (MQ=255)gCACATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGa < 1:701949/71‑1 (MQ=255) caTCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGaaa < 1:2627166/70‑1 (MQ=255) aTCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTTaaa < 1:2694288/69‑1 (MQ=255) ctttctttGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTc > 1:1165722/1‑71 (MQ=255) tctttGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAg > 1:812388/1‑71 (MQ=255) ctttGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAg < 1:1050495/70‑1 (MQ=255) tGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGt > 1:812827/1‑63 (MQ=255) tGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTa < 1:943246/69‑1 (MQ=255) tGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTa < 1:917947/69‑1 (MQ=255) cTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGt < 1:1442689/70‑1 (MQ=255) agaTCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTACt < 1:2567991/71‑1 (MQ=255) tCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTc < 1:2106670/71‑1 (MQ=255) cGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATa > 1:2143086/1‑52 (MQ=255) tttGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATAGTGAAATAGTCCAGt < 1:1499535/53‑1 (MQ=255) ttGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACg > 1:681074/1‑71 (MQ=255) ttGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATAGTGAAATAGTCCAGTATGTTCCTTTCGATAAACg > 1:430288/1‑71 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTc > 1:1758072/1‑57 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCt > 1:2722102/1‑59 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGt > 1:1693611/1‑71 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGt > 1:1405592/1‑71 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGt > 1:2420721/1‑71 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGt > 1:2371070/1‑71 (MQ=255) tGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGt > 1:1812629/1‑71 (MQ=255) cgcgTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTg < 1:697516/70‑1 (MQ=255) gcgTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgca < 1:675972/71‑1 (MQ=255) tctcCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGa < 1:1193232/58‑1 (MQ=255) tctcCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatgg < 1:732704/71‑1 (MQ=255) tcCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTg > 1:1200763/1‑64 (MQ=255) tcCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCAAGTATGTTCCTTTCg > 1:786470/1‑55 (MQ=255) tcCGCTCACTGTTTGATTCGCCGTGATAGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatggca > 1:2667650/1‑71 (MQ=255) tcCGCTCACTGTTTGATTCGCCGTGATAGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatggca > 1:66227/1‑71 (MQ=255) cGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGt < 1:1010887/45‑1 (MQ=255) cGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgca > 1:1324885/1‑64 (MQ=255) cTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTg < 1:2811936/56‑1 (MQ=255) tGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCtt < 1:2739056/43‑1 (MQ=255) tGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCtt < 1:1501463/43‑1 (MQ=255) tGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCtt < 1:867052/43‑1 (MQ=255) tGATTCGCCGTGATAGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGtctc < 1:739790/70‑1 (MQ=255) gATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatggca > 1:1176800/1‑57 (MQ=255) gATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgca < 1:1471146/52‑1 (MQ=255) gATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGtctct < 1:1182741/70‑1 (MQ=255) gATTCGCCGTGATAGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatgg < 1:2547492/55‑1 (MQ=255) aTTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatggc < 1:1850183/55‑1 (MQ=255) tCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatggcat > 1:1404847/1‑55 (MQ=255) ccGTTATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatgg < 1:1166272/49‑1 (MQ=255) cGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCa < 1:1829094/71‑1 (MQ=255) aTGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatg < 1:2602802/43‑1 (MQ=255) aTGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTgcatg < 1:755462/43‑1 (MQ=255) | GCACATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGTGATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCA > W3110S.gb/118910‑119040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |