| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 707,271 | C→T | 14.2% | H253Y (CAC→TAC) | glnS → | glutamyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 707,271 | 0 | C | T | 14.2% | 92.6 / 6.2 | 35 | H253Y (CAC→TAC) | glnS | glutamyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (18/12); new base T (2/3); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGA > W3110S.gb/707206‑707337 | cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCcg > 1:2418529/1‑71 (MQ=255) ccAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTAt > 1:1428400/1‑70 (MQ=255) cAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATg < 1:488424/70‑1 (MQ=255) cAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATg < 1:1605744/70‑1 (MQ=255) cAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATg < 1:2207233/70‑1 (MQ=255) ggACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTa > 1:2197079/1‑66 (MQ=255) cAACGGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTtct < 1:1757718/71‑1 (MQ=255) cgtcgtCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTcgcg < 1:1036217/71‑1 (MQ=255) cgtcgtCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTcgcg < 1:1039423/71‑1 (MQ=255) tcgtcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAg < 1:2460753/61‑1 (MQ=255) gtcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAGtt > 1:492308/1‑61 (MQ=255) gtcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCAc > 1:257214/1‑44 (MQ=255) cTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGaa > 1:1430545/1‑71 (MQ=255) tGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGaa > 1:2048521/1‑70 (MQ=255) tACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATc < 1:27586/70‑1 (MQ=255) tACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATc < 1:1067853/70‑1 (MQ=255) aCGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTg > 1:1038297/1‑71 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTa > 1:1287517/1‑48 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTa > 1:2655486/1‑48 (MQ=255) ggTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATaca < 1:2525603/71‑1 (MQ=255) tGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa < 1:1060413/62‑1 (MQ=255) gACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTg < 1:966285/69‑1 (MQ=255) gACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGAt > 1:415367/1‑71 (MQ=255) gACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGAt > 1:412084/1‑71 (MQ=255) gACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGAt > 1:408953/1‑71 (MQ=255) gACAAAATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGa > 1:2045043/1‑70 (MQ=255) aacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGaa > 1:712843/1‑50 (MQ=255) aacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGaa > 1:669844/1‑50 (MQ=255) aTCACGATTCCTGTTTACCCGCGCCAGTATGATTTCTCGCGCCTGAATCTGGaa > 1:372658/1‑54 (MQ=255) aTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTcc > 1:1911837/1‑69 (MQ=255) tCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa < 1:1383212/53‑1 (MQ=255) cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATaca > 1:40102/1‑56 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCg > 1:1483136/1‑71 (MQ=255) cTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTg < 1:183839/71‑1 (MQ=255) ttCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGa < 1:1107121/69‑1 (MQ=255) cACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGa > 1:1241132/1‑49 (MQ=255) cACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGa > 1:1507453/1‑49 (MQ=255) | CGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGA > W3110S.gb/707206‑707337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |