| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,903,549 | T→C | 10.9% | S104P (TCA→CCA) | bax → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,903,549 | 0 | T | C | 10.9% | 140.7 / 10.0 | 55 | S104P (TCA→CCA) | bax | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/20); new base C (4/2); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903484‑3903618 | ggACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCaaaa < 1:2044989/71‑1 (MQ=255) ccGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAg < 1:2811176/71‑1 (MQ=255) ccGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAg < 1:2213009/71‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt < 1:2653526/71‑1 (MQ=255) tGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAg < 1:1706830/71‑1 (MQ=255) ccTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAggg > 1:1799834/1‑71 (MQ=255) ccTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGTATCAggg > 1:1058506/1‑71 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAg < 1:1974441/59‑1 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:794773/1‑71 (MQ=255) cATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa > 1:1242656/1‑60 (MQ=255) cATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa < 1:2715050/70‑1 (MQ=255) aTTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa > 1:1053927/1‑59 (MQ=255) aTTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa > 1:1081886/1‑59 (MQ=255) aTTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCaa < 1:671409/52‑1 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATgg < 1:240979/71‑1 (MQ=255) tACCAGCCAAAATG‑GGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:1716557/1‑70 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAg > 1:1220815/1‑60 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAg > 1:1706356/1‑60 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAg > 1:1669841/1‑60 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:979830/1‑66 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:742955/1‑70 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGTa > 1:1126488/1‑56 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:745764/70‑1 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCa < 1:270191/71‑1 (MQ=255) gCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGt < 1:2417679/51‑1 (MQ=255) ccAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg > 1:2272182/1‑71 (MQ=255) ccAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg > 1:1893790/1‑71 (MQ=255) cAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:2428533/1‑51 (MQ=255) cAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:1642966/1‑51 (MQ=255) aaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGt < 1:377291/47‑1 (MQ=255) gCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCg > 1:2699275/1‑71 (MQ=255) gCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCg > 1:2702459/1‑71 (MQ=255) cATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:2346183/1‑49 (MQ=255) cATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:24676/1‑49 (MQ=255) aTTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGcgcg < 1:152467/71‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc < 1:2274634/45‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:1290794/52‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:1414854/52‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:1475082/52‑1 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATc < 1:63188/38‑1 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTc > 1:190380/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCAc > 1:1150750/1‑52 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:1053985/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:1863659/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:2696584/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATAAGGGCCAATGGTCa > 1:605597/1‑51 (MQ=255) aCTGCGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:2671618/1‑71 (MQ=255) gAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:1768655/1‑71 (MQ=255) aaCGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:1861576/71‑1 (MQ=255) tAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg < 1:2565324/44‑1 (MQ=255) tAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg < 1:565954/44‑1 (MQ=255) aaCTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGc > 1:1179878/1‑71 (MQ=255) aaCTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGc > 1:1149509/1‑71 (MQ=255) aCTGGTTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGc > 1:1537715/1‑70 (MQ=255) ggCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCaaa > 1:2346783/1‑71 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAg < 1:631872/38‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAg < 1:97789/38‑1 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGaa > 1:882350/1‑50 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACAt > 1:806492/1‑56 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACAt > 1:2720731/1‑56 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCACACGCTAc > 1:1604416/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAAc > 1:809514/1‑64 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:1236939/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:1870693/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:1589430/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:1223818/70‑1 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:945269/70‑1 (MQ=255) | GGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903484‑3903618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |