| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,958,682 | C→T | 12.2% | A69V (GCC→GTC) | yhjJ → | predicted zinc‑dependent peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,958,682 | 0 | C | T | 12.2% | 115.7 / 6.1 | 41 | A69V (GCC→GTC) | yhjJ | predicted zinc‑dependent peptidase |
| Reads supporting (aligned to +/- strand): ref base C (17/19); new base T (2/3); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGACCACCCCCCAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGCGGTGGCC > W3110S.gb/3958615‑3958750 | cTGACCACCCCCCAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGa < 1:2140372/71‑1 (MQ=255) tGACCACCCCCCAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGa < 1:2405611/70‑1 (MQ=255) accaccCCCCAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGaa < 1:2236632/69‑1 (MQ=255) cccccAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTacac < 1:1263939/71‑1 (MQ=255) gCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACacaaca > 1:2192850/1‑69 (MQ=255) gCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACacaaca > 1:2263816/1‑69 (MQ=255) gTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGc > 1:1635701/1‑71 (MQ=255) gTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGc > 1:140725/1‑71 (MQ=255) tCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCg < 1:1470319/71‑1 (MQ=255) tCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCg < 1:386049/71‑1 (MQ=255) cGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTa > 1:2388364/1‑69 (MQ=255) cGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGTCGAAAGTACACAACAGAGCGGTTACAGTCa > 1:2126181/1‑71 (MQ=255) tgtTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACg < 1:1611095/71‑1 (MQ=255) ttGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGa < 1:1157347/40‑1 (MQ=255) ttGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGc < 1:1392364/70‑1 (MQ=255) ttGAAATTCGCCTGCTCGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGc < 1:1348158/70‑1 (MQ=255) gAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCAt > 1:2485462/1‑71 (MQ=255) gAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCAt > 1:200406/1‑71 (MQ=255) aaaTTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAg > 1:1661711/1‑61 (MQ=255) aaaTTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAg > 1:1658513/1‑61 (MQ=255) cGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACa > 1:1904859/1‑55 (MQ=255) ccTGCTGGTTAAATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCg < 1:988793/70‑1 (MQ=255) ccTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCg < 1:2209205/69‑1 (MQ=255) ccTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCg < 1:1894278/69‑1 (MQ=255) ccTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCCGTAACAGTCACGCCATCCCTCGTa > 1:1721120/1‑71 (MQ=255) ggTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTgcg > 1:465394/1‑70 (MQ=255) gTT‑AATACCGGTTCGCTCGTCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATc < 1:2241334/57‑1 (MQ=255) gTT‑AATACCGGTTCGCTCGTCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATc < 1:2761439/57‑1 (MQ=255) tt‑AATACCGGTTCCCTCGCCGAAAGTACACAACaga < 1:1220298/36‑1 (MQ=255) aaTACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAAc > 1:941834/1‑71 (MQ=255) aTACCGGTTCGCTCGTCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACg > 1:294849/1‑71 (MQ=255) tACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGtt < 1:2224328/38‑1 (MQ=255) tACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGtt < 1:2227399/38‑1 (MQ=255) tACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGc < 1:887385/48‑1 (MQ=255) aCCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACg > 1:557493/1‑69 (MQ=255) ccGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTAt > 1:2505073/1‑58 (MQ=255) ccGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTAt > 1:2305572/1‑58 (MQ=255) ggTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATCGCGCTAAc > 1:566787/1‑65 (MQ=255) gTTCGCTCGTCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATc < 1:2587256/47‑1 (MQ=255) ttCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGc < 1:2721912/70‑1 (MQ=255) ttCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGc < 1:1246665/70‑1 (MQ=255) tcgctcgcCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTa > 1:1713211/1‑52 (MQ=255) tcgctcgcCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTa > 1:176130/1‑52 (MQ=255) cgctcgtCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATcc > 1:1926570/1‑45 (MQ=255) cgctcgcCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTAtt > 1:1712396/1‑53 (MQ=255) ctcgtCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGCGGTgg < 1:678802/71‑1 (MQ=255) cgcCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGCGGTGGcc > 1:2400685/1‑71 (MQ=255) | CTGACCACCCCCCAGCGTCCCAGCGATCGTGTTGAAATTCGCCTGCTGGTT‑AATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCCATCCCTCGTATTGCGCTAACGCAAAGCGGTGGCC > W3110S.gb/3958615‑3958750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |