| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,000,806 | A→T | 7.8% | D37V (GAT→GTT) | yhiO → | predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,000,806 | 0 | A | T | 7.8% | 137.7 / 4.3 | 51 | D37V (GAT→GTT) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (18/29); new base T (2/2); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAA > W3110S.gb/4000739‑4000870 | tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc > 1:1875032/1‑70 (MQ=255)tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1315702/70‑1 (MQ=255) gCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATcc < 1:1263246/70‑1 (MQ=255) cATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAt < 1:2130704/71‑1 (MQ=255) aTTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAtt > 1:2062866/1‑71 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTc < 1:2788558/66‑1 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:159388/71‑1 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:484233/71‑1 (MQ=255) aTGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc > 1:2386371/1‑70 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:706107/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:884052/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:927035/71‑1 (MQ=255) gcgcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:2688235/1‑71 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1664218/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1975668/71‑1 (MQ=255) cTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:679075/70‑1 (MQ=255) tATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:2200052/70‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:67230/61‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:1741403/61‑1 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTg > 1:2630857/1‑50 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg > 1:191405/1‑69 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:918734/70‑1 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg > 1:1716771/1‑69 (MQ=255) aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCt > 1:1681067/1‑71 (MQ=255) cTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtt < 1:1249368/71‑1 (MQ=255) tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAat < 1:383643/51‑1 (MQ=255) tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAatat < 1:2798599/53‑1 (MQ=255) tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:755496/60‑1 (MQ=255) cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCAt > 1:1649489/1‑37 (MQ=255) cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTCCTCTATCAATATGTTGATGGAGGGGGCttttt > 1:2803839/1‑71 (MQ=255) cgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:405410/52‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGctc < 1:1079988/36‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGctc < 1:583481/36‑1 (MQ=255) gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:995583/70‑1 (MQ=255) tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1174885/41‑1 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:2688404/1‑41 (MQ=255) aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:1818551/1‑43 (MQ=255) aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:538026/1‑43 (MQ=255) aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:1500247/1‑43 (MQ=255) cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAc < 1:1136115/56‑1 (MQ=255) cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAc < 1:974372/56‑1 (MQ=255) cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACATCACATGGccaacc > 1:1529245/1‑71 (MQ=255) tGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:1678012/40‑1 (MQ=255) tGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:978650/40‑1 (MQ=255) gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAg > 1:55252/1‑41 (MQ=255) cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaacccaa > 1:1203351/1‑71 (MQ=255) gTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc < 1:341069/68‑1 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAg > 1:1010480/1‑36 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGTCTTTTTTACCTCACATGGCCAACCcaacaa > 1:1569033/1‑70 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:2545317/71‑1 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa > 1:946721/1‑71 (MQ=255) | TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAA > W3110S.gb/4000739‑4000870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |