| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 710,341 | C→T | 68.0% | P44S (CCA→TCA) | ybfN → | predicted lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 710,341 | 0 | C | T | 68.0% | 25.7 / 19.5 | 25 | P44S (CCA→TCA) | ybfN | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base T (11/6); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAG > W3110S.gb/710286‑710406 | aGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAg > 1:2562520/1‑71 (MQ=255) aaaGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCg < 1:2434170/70‑1 (MQ=255) gagGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCgtgt > 1:1235659/1‑70 (MQ=255) tACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACg < 1:420016/70‑1 (MQ=255) cAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAAt > 1:635612/1‑42 (MQ=255) cAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGa > 1:1478134/1‑45 (MQ=255) cTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTTAAGCCTGCCAGAGCGTGTTAAACg < 1:1018718/62‑1 (MQ=255) tGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCag < 1:1397821/48‑1 (MQ=255) gTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaag > 1:87083/1‑68 (MQ=255) tATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaaga > 1:2734669/1‑68 (MQ=255) tATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaaga > 1:1811033/1‑68 (MQ=255) aTCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa > 1:429592/1‑69 (MQ=255) tCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa > 1:1166778/1‑68 (MQ=255) tCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa > 1:1726444/1‑68 (MQ=255) tCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa < 1:642895/35‑1 (MQ=255) tCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa > 1:422299/1‑68 (MQ=255) cAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGc > 1:598428/1‑40 (MQ=255) aaCACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa > 1:1213009/1‑66 (MQ=255) aaCACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAgaga < 1:2307109/70‑1 (MQ=255) cacCGCACAAGGTTCGCCAGAAAAATTTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAaac < 1:516239/71‑1 (MQ=255) ggTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcag > 1:205069/1‑69 (MQ=255) ggTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcag > 1:1323588/1‑69 (MQ=255) gTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcagcag < 1:1634733/71‑1 (MQ=255) gTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcag < 1:1692368/68‑1 (MQ=255) gTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcag < 1:1392830/68‑1 (MQ=255) | AGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAG > W3110S.gb/710286‑710406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |