| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,042,971 | G→A | 85.2% | A39T (GCC→ACC) | livK → | leucine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,042,971 | 0 | G | A | 85.2% | 63.3 / 5.3 | 27 | A39T (GCC→ACC) | livK | leucine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (8/15); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCAA > W3110S.gb/4042908‑4043032 | tCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTg > 1:1829632/1‑71 (MQ=255) acCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGa < 1:349719/71‑1 (MQ=255) tGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATgg < 1:1937848/69‑1 (MQ=255) gCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAAtt > 1:2510539/1‑71 (MQ=255) cTGACGATATTAAAGTTGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAAttt < 1:284588/71‑1 (MQ=255) cGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATgg < 1:2268330/62‑1 (MQ=255) atTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTaa > 1:2649601/1‑65 (MQ=255) aaaGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAAtt > 1:1452034/1‑59 (MQ=255) aaaGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGcgcgcg > 1:1162656/1‑71 (MQ=255) aaaGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGcgcgcg > 1:1903984/1‑71 (MQ=255) tCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAAttt < 1:794082/56‑1 (MQ=255) gCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGc < 1:1579807/71‑1 (MQ=255) ccGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGc < 1:2300967/70‑1 (MQ=255) ttGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGata < 1:2408220/42‑1 (MQ=255) ttGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGa < 1:229519/40‑1 (MQ=255) gTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATg > 1:858901/1‑42 (MQ=255) gTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTa > 1:2784449/1‑70 (MQ=255) ttgcgcGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAAttt < 1:1679638/44‑1 (MQ=255) ggCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa > 1:85006/1‑71 (MQ=255) cgATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt < 1:1595081/71‑1 (MQ=255) aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt < 1:2534710/69‑1 (MQ=255) aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt < 1:1438957/69‑1 (MQ=255) aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt < 1:1379580/69‑1 (MQ=255) cGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGcc > 1:512917/1‑70 (MQ=255) ggCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCaa < 1:1853873/71‑1 (MQ=255) ggCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCaa < 1:2554735/71‑1 (MQ=255) ggCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCaa < 1:1312968/71‑1 (MQ=255) | TCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCAA > W3110S.gb/4042908‑4043032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |