| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,057,014 | A→G | 11.4% | I121I (ATT→ATC) | yrhA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,057,014 | 0 | A | G | 11.4% | 109.2 / 7.4 | 44 | I121I (ATT→ATC) | yrhA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/17); new base G (3/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATCATCATTAT > W3110S.gb/4056945‑4057075 | cAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGaaa > 1:2701705/1‑71 (MQ=255)cAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGaaa > 1:180572/1‑71 (MQ=255)cAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGaaa > 1:1754145/1‑71 (MQ=255) aaCACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCt < 1:2536395/71‑1 (MQ=255) aaCACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCt < 1:1237476/71‑1 (MQ=255) acCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCtata < 1:648959/71‑1 (MQ=255) acCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCta < 1:1995717/69‑1 (MQ=255) catACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATccc > 1:1614935/1‑71 (MQ=255) catACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATccc > 1:2341300/1‑71 (MQ=255) aaaTCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAGATGCTATAATCCCCGATCa > 1:1564453/1‑69 (MQ=255) aaGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCg > 1:430889/1‑68 (MQ=255) aGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCt < 1:1416088/71‑1 (MQ=255) aGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCt < 1:1131049/71‑1 (MQ=255) ttGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATaa > 1:654028/1‑48 (MQ=255) tGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCta > 1:2394837/1‑44 (MQ=255) tGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCta > 1:275610/1‑44 (MQ=255) tGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCta > 1:2266849/1‑44 (MQ=255) tGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGAt > 1:2083787/1‑55 (MQ=255) tGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTg > 1:1520742/1‑70 (MQ=255) agcaTCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTg > 1:860401/1‑66 (MQ=255) aTCACCTTTAATGTCATAAGTAAATATTGAAATGCtat < 1:2491659/38‑1 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCtat < 1:1625690/37‑1 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCg < 1:228993/45‑1 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAAcc > 1:557141/1‑55 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCt < 1:2742071/60‑1 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCt < 1:414293/60‑1 (MQ=255) tCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCt < 1:1082014/60‑1 (MQ=255) aCCTTTAATGTCATAAGTAAATATTGAAATGCtata > 1:2732240/1‑36 (MQ=255) aCCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTaa > 1:1088625/1‑51 (MQ=255) aCCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAAc > 1:479812/1‑52 (MQ=255) tAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTa > 1:1464608/1‑57 (MQ=255) tAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTAt > 1:2063362/1‑70 (MQ=255) tAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTAt > 1:1246990/1‑70 (MQ=255) aaTGTCATAAGTAAATATTGAGATGCTATAATCCCCGATCACTAACCGTTCTTg > 1:644685/1‑54 (MQ=255) aaTGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTg > 1:354037/1‑54 (MQ=255) tGTCATAAGTAAATATTGAGATGCTATAATCCCCGAt < 1:1643048/37‑1 (MQ=255) tGTCATAAGTAAATATTGAGATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGaa < 1:2223388/70‑1 (MQ=255) tCATAAGTAAATATTGAGATGCTATAATCCCCGATCa > 1:1819948/1‑37 (MQ=255) tCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAatc < 1:455031/71‑1 (MQ=255) aTAAGTAAATATTGAAATGCTATAATCCCCGATCACTACCCGTTCTTGTAGATCAGGGTTTATGAAat > 1:2414284/1‑68 (MQ=255) aGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATcatcat > 1:1998210/1‑71 (MQ=255) aGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATcatcat < 1:1830050/71‑1 (MQ=255) tAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATcatcat < 1:84899/69‑1 (MQ=255) aaaTATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATCATCattat > 1:287195/1‑71 (MQ=255) aaTATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATc < 1:2321200/47‑1 (MQ=255) aaTATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATcatcat > 1:868913/1‑67 (MQ=255) atatTGAAATGCTATAATCCCCGATCACTAACCGTTCTTg < 1:1548228/40‑1 (MQ=255) | CAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTTTAATGTCATAAGTAAATATTGAAATGCTATAATCCCCGATCACTAACCGTTCTTGTAGATCAGGGTTTATGAAATCATCATTAT > W3110S.gb/4056945‑4057075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |