| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,064,053 | T→A | 39.9% | I233N (ATC→AAC) | gntU → | gluconate transporter, low affinity GNT 1 system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,064,053 | 0 | T | A | 39.9% | 21.8 / 29.4 | 30 | I233N (ATC→AAC) | gntU | gluconate transporter, low affinity GNT 1 system |
| Reads supporting (aligned to +/- strand): ref base T (13/5); new base A (8/4); total (21/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACGACATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCAA > W3110S.gb/4063985‑4064118 | cgacgacATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAAcc < 1:2222637/70‑1 (MQ=255) gacgacATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCct > 1:2431013/1‑71 (MQ=255) acATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCctgc > 1:2602071/1‑69 (MQ=255) cATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCtgctgc > 1:1139394/1‑71 (MQ=255) aGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCgct > 1:563866/1‑71 (MQ=255) cGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCgctgg > 1:1824650/1‑71 (MQ=255) tctcGGCGAAGGCAAAATGCCATCTTTCTGATTCAGCCTGTCGATGATCCTGCTGCCgctggt < 1:1913468/63‑1 (MQ=255) tcGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCtgctgc > 1:1947299/1‑54 (MQ=255) tcGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCtgctgc > 1:1085270/1‑54 (MQ=255) tcGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTa > 1:2659821/1‑68 (MQ=255) tcGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTCTCGCTGATCCtgctgc > 1:1993808/1‑54 (MQ=255) gCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCgctggtgct < 1:1595565/61‑1 (MQ=255) ggCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATcc > 1:2338070/1‑40 (MQ=255) ggCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAAc > 1:1375831/1‑71 (MQ=255) cAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAAc < 1:603528/69‑1 (MQ=255) aaaaTGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg > 1:1783301/1‑58 (MQ=255) aaaaTGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCAt > 1:2763502/1‑71 (MQ=255) aTCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCcgcgc > 1:1608295/1‑71 (MQ=255) tCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCAt > 1:1721227/1‑62 (MQ=255) tCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCAt > 1:1717933/1‑62 (MQ=255) tCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCgctgg < 1:2284186/40‑1 (MQ=255) cTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTa > 1:289229/1‑47 (MQ=255) tttCGGATTCAGCCTGTCGCTGAACCTGCTGCCgctg > 1:2683474/1‑37 (MQ=255) ggATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTtg < 1:874483/70‑1 (MQ=255) aTTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTtg < 1:13177/68‑1 (MQ=255) aGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAAc < 1:1558737/47‑1 (MQ=255) tCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGAt > 1:1881699/1‑70 (MQ=255) cGCTGAACCTGGTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAgg > 1:2269642/1‑67 (MQ=255) gCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCaa > 1:562271/1‑71 (MQ=255) aTCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCa < 1:2093908/66‑1 (MQ=255) | TGACGACATCAGCGAACCGCATCTCGGCGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCAA > W3110S.gb/4063985‑4064118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |