Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,099,912 C→T 86.3% A34T (GCT→ACT)  feoB ← fused ferrous iron transporter, protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,099,9120CT86.3% 122.9 / 13.2 51A34T (GCT→ACT) feoBfused ferrous iron transporter, protein B
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base T (19/25);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCCACCAGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAATTTGG  >  W3110S.gb/4099845‑4099978
                                                                   |                                                                  
tCCACCAGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCa                                                                 <  1:794455/71‑1 (MQ=255)
   accaGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:1101060/71‑1 (MQ=255)
    ccaGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCa                                                                 <  1:757398/67‑1 (MQ=255)
    ccaGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTa                                                             >  1:1415160/1‑71 (MQ=255)
      aGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTAcc                                                           >  1:1989030/1‑71 (MQ=255)
      aGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTAcc                                                           >  1:186259/1‑71 (MQ=255)
       gCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCa                                                                 <  1:1096655/64‑1 (MQ=255)
       gCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCa                                                                 <  1:1379924/64‑1 (MQ=255)
         gTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTACCTa                                                         >  1:2607339/1‑70 (MQ=255)
            aCCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:1901560/62‑1 (MQ=255)
            aCCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:2739042/62‑1 (MQ=255)
              cTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCt                                                   <  1:2040214/71‑1 (MQ=255)
                gatgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTa                                                             >  1:1918887/1‑59 (MQ=255)
                gatgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTa                                                             >  1:2351280/1‑59 (MQ=255)
                gatgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTa                                                             >  1:2704809/1‑59 (MQ=255)
                  tgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:1899641/56‑1 (MQ=255)
                  tgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:1970524/56‑1 (MQ=255)
                  tgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGtt                                                              <  1:1255736/56‑1 (MQ=255)
                  tgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACg                                               >  1:2479172/1‑71 (MQ=255)
                  tgatCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACg                                               >  1:1392223/1‑71 (MQ=255)
                        gtggtgGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGcc                                         <  1:1170856/71‑1 (MQ=255)
                        gtggtgGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGcc                                         <  1:871271/71‑1 (MQ=255)
                        gtggtgGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGcc                                         <  1:2448950/71‑1 (MQ=255)
                        gtggtgGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTACCTACACGCTGACGTGAGcc                                         <  1:1339132/71‑1 (MQ=255)
                           gtggAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAg                                       <  1:2737961/70‑1 (MQ=255)
                            tggAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTg                                     <  1:952808/71‑1 (MQ=255)
                                 aaTTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTACCTACACGCt                                                   <  1:1969078/52‑1 (MQ=255)
                                     gCCCTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTa                                                         >  1:454299/1‑42 (MQ=255)
                                      cccTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTaa                           <  1:1992601/71‑1 (MQ=255)
                                      cccTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTa                            <  1:2528298/70‑1 (MQ=255)
                                      cccTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTa                            <  1:2562325/70‑1 (MQ=255)
                                      cccTTCTTTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTa                            <  1:2636774/70‑1 (MQ=255)
                                         ttcttTACGTTCGACGGTAACGCCAGTCCAGTTAc                                                            >  1:2627584/1‑35 (MQ=255)
                                           cttTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATa                        <  1:2425990/69‑1 (MQ=255)
                                           cttTACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAAc                      >  1:629531/1‑71 (MQ=255)
                                            tttACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACg                     >  1:1604730/1‑71 (MQ=255)
                                              tACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAAc                      <  1:1606154/68‑1 (MQ=255)
                                              tACGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAAc                      >  1:904746/1‑68 (MQ=255)
                                               aCGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGtt                   <  1:1544168/70‑1 (MQ=255)
                                                cGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGt                 <  1:718501/71‑1 (MQ=255)
                                                cGTTCGACGGTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGt                 <  1:894255/71‑1 (MQ=255)
                                                         gTAACGCCAGTCCAGTTACCTACACGCTGACGTGAGcc                                         <  1:379738/38‑1 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAg                                       >  1:432695/1‑38 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAg                                       >  1:1511560/1‑38 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTg             >  1:2274552/1‑64 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGaa       >  1:998922/1‑70 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAAt      >  1:84403/1‑71 (MQ=255)
                                                           aaCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAAt      >  1:2331052/1‑71 (MQ=255)
                                                           aaCGCCAGCCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAAt      >  1:1290766/1‑71 (MQ=255)
                                                            aCGCCAGTCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAAtt     >  1:905607/1‑71 (MQ=255)
                                                               ccagtccagtTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAATTTg   >  1:1556337/1‑70 (MQ=255)
                                                               ccAGCCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAATTTgg  <  1:35395/71‑1 (MQ=255)
                                                                   |                                                                  
TCCACCAGCGTGACCTGATGATCGGTGGTGGAGAATTGCCCTTCTTTACGTTCGACGGTAACGCCAGCCCAGTTACCTACACGCTGACGTGAGCCAGTGAGCTGGTTAAATAACGTTGTCTTGCCAGAATTTGG  >  W3110S.gb/4099845‑4099978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: