| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,103,846 | T→C | 86.5% | Y5H (TAC→CAC) | ompR → | DNA‑binding response regulator in two‑component regulatory system with EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,103,846 | 0 | T | C | 86.5% | 99.6 / 7.1 | 37 | Y5H (TAC→CAC) | ompR | DNA‑binding response regulator in two‑component regulatory system with EnvZ |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (20/12); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGA > W3110S.gb/4103792‑4103910 | tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGa < 1:1821057/61‑1 (MQ=255) cacGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTc > 1:1846197/1‑70 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGAt < 1:2608200/71‑1 (MQ=255) gCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGAt < 1:1869326/70‑1 (MQ=255) tACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGAt < 1:616255/67‑1 (MQ=255) aCAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgac < 1:2380200/69‑1 (MQ=255) cAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga < 1:404033/70‑1 (MQ=255) cAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGAt > 1:531166/1‑65 (MQ=255) taaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgacga < 1:2509526/68‑1 (MQ=255) aaaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAAACACAAGATTCTGGTGGTCGATgacga < 1:1303694/69‑1 (MQ=255) gttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcgc > 1:689269/1‑71 (MQ=255) ccTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACa > 1:361299/1‑58 (MQ=255) gTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgacga < 1:994123/47‑1 (MQ=255) tACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgacg < 1:1472985/45‑1 (MQ=255) tACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTgcgc < 1:2524765/63‑1 (MQ=255) acaaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATgacg < 1:2075261/44‑1 (MQ=255) acaaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATTACGACATGCGCCTGCGTGCGCTGCTGGaa > 1:1472064/1‑70 (MQ=255) acaaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACAt > 1:15664/1‑48 (MQ=255) acaaacaaTGCAAGAGAACCACAAGATTCAGGTGGTCGATGACGACa > 1:2272093/1‑47 (MQ=39) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:394406/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:464006/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:1996107/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:107207/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:1971167/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:1406028/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:1285606/1‑71 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg > 1:1168915/1‑71 (MQ=255) aacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACAt > 1:1931602/1‑45 (MQ=255) aacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCgctgc > 1:1090723/1‑62 (MQ=255) aacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGt > 1:1758449/1‑70 (MQ=255) tGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAtct > 1:482500/1‑70 (MQ=255) gCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCa > 1:2142046/1‑71 (MQ=255) agagAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGtt < 1:1583947/62‑1 (MQ=255) gagaACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCATGCGCTGCTGGAACGTTATCTCACCGa < 1:2422318/71‑1 (MQ=255) gaACCACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcg > 1:1368903/1‑42 (MQ=255) gaACCACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcg > 1:628810/1‑42 (MQ=255) cTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGtt < 1:1801526/56‑1 (MQ=255) | TACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGA > W3110S.gb/4103792‑4103910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |