| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,125,813 | C→T | 15.8% | P152L (CCT→CTT) | rpe → | D‑ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,125,813 | 0 | C | T | 15.8% | 146.0 / 17.9 | 57 | P152L (CCT→CTT) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (23/25); new base T (4/5); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125745‑4125881 | gATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCt > 1:1353951/1‑70 (MQ=255) aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:490249/71‑1 (MQ=255) ggATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:1162674/1‑71 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGTTCAGTCTTTCATTCTTCa < 1:287901/68‑1 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa > 1:1144623/1‑70 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa < 1:84542/70‑1 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:1640912/1‑70 (MQ=255) tGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:1886200/61‑1 (MQ=255) tGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:1796451/61‑1 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1245703/70‑1 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1342803/70‑1 (MQ=255) tgtgATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAAc > 1:1055943/1‑71 (MQ=255) aTCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTgcg > 1:1871781/1‑71 (MQ=255) ccTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGa > 1:2056424/1‑60 (MQ=255) ccTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTgcg > 1:2807724/1‑69 (MQ=255) ctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1020393/1‑70 (MQ=255) aTGTCCGTCAACCCTGGTTTCGGCTGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACg > 1:638626/1‑71 (MQ=39) tGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:2741623/43‑1 (MQ=255) tGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:1297565/43‑1 (MQ=255) gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:119100/38‑1 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGcgcg < 1:363953/57‑1 (MQ=255) cAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:2182687/1‑38 (MQ=255) cAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTa > 1:2557778/1‑71 (MQ=255) cAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTa > 1:782445/1‑71 (MQ=255) aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:2341350/1‑71 (MQ=255) aCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATc < 1:789649/71‑1 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTg > 1:526733/1‑38 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTg > 1:491624/1‑38 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:2136599/71‑1 (MQ=255) ggTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:589329/1‑69 (MQ=255) ggTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:2113886/1‑69 (MQ=255) gattCGGCGGTCAGTCTTTCATTACTCAAACACTGGATaa < 1:199509/38‑1 (MQ=255) gTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:477700/1‑53 (MQ=255) ggTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCtt < 1:1087769/71‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAg < 1:2231418/59‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:2500366/70‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:1366465/70‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:907243/70‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:2322879/38‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGt < 1:1674568/39‑1 (MQ=255) tCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1273981/54‑1 (MQ=255) tCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1758384/54‑1 (MQ=255) tCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1360532/54‑1 (MQ=255) tCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAg < 1:126327/57‑1 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:1047752/66‑1 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:945801/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:1732644/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGt > 1:1815069/1‑54 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc < 1:1046696/69‑1 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc < 1:1286797/69‑1 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:278775/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:1327470/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:1404454/1‑71 (MQ=255) cATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc > 1:1327503/1‑71 (MQ=255) cATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc > 1:526274/1‑71 (MQ=255) aTTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc < 1:2476698/70‑1 (MQ=255) aTTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc < 1:2473573/70‑1 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcga > 1:2784624/1‑44 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGTCTTTGACATTCGACTAg < 1:1792412/70‑1 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:1409203/1‑66 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg > 1:927307/1‑70 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg > 1:328074/1‑69 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg > 1:1025215/1‑69 (MQ=255) | GATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125745‑4125881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |