| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,184,035 | C→A | 84.6% | G3* (GGA→TGA) R269M (AGG→ATG) |
gspD ← gspC ← |
general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,035 | 0 | C | A | 84.6% | 66.3 / 14.5 | 39 | G3* (GGA→TGA) R269M (AGG→ATG) | gspD gspC | general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base A (16/17); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCC > W3110S.gb/4183973‑4184102 | aGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:1504436/69‑1 (MQ=255) tgtCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:389504/65‑1 (MQ=255) tgtCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCa < 1:1798826/71‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcatt > 1:2253851/1‑71 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:948976/71‑1 (MQ=255) aaGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:436775/52‑1 (MQ=255) ggCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCa > 1:122500/1‑52 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTg > 1:2319639/1‑70 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCattcattc > 1:1061858/1‑65 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCca > 1:846580/1‑58 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCca > 1:1899451/1‑58 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:125958/1‑69 (MQ=255) gtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:1567945/71‑1 (MQ=255) gtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACg < 1:2304045/47‑1 (MQ=255) tgctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGtt < 1:1638466/69‑1 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc > 1:1148691/1‑71 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc < 1:1165596/71‑1 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGtt > 1:2039713/1‑62 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc < 1:1455201/66‑1 (MQ=255) aagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGaaa < 1:2334358/71‑1 (MQ=255) aagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGaaa < 1:485712/71‑1 (MQ=255) gcagTTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAttt < 1:302223/71‑1 (MQ=255) tGATTTTATTGAGTCATTTCATGACGCAGTCcattc < 1:2512558/36‑1 (MQ=255) tGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:772515/47‑1 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCcattca > 1:2458113/1‑36 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCattcattc > 1:2050617/1‑39 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTcc > 1:2307403/1‑40 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTa > 1:1529587/1‑66 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTAt > 1:2578533/1‑67 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:488543/1‑71 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:2411942/1‑71 (MQ=255) aTTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGtt > 1:533151/1‑47 (MQ=255) aTTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg < 1:2776280/70‑1 (MQ=255) aTTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg < 1:579464/70‑1 (MQ=255) aTTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg < 1:23435/70‑1 (MQ=255) tttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:698161/1‑71 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCggg > 1:2227951/1‑71 (MQ=255) tATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTa < 1:859716/39‑1 (MQ=255) gTCATTTCATGACGCAGTCAATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGcc < 1:501436/71‑1 (MQ=255) | AGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCC > W3110S.gb/4183973‑4184102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |