| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,208,832 | A→G | 13.6% | H126R (CAC→CGC) | yrdD → | predicted DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,208,832 | 0 | A | G | 13.6% | 106.9 / 10.5 | 44 | H126R (CAC→CGC) | yrdD | predicted DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (3/3); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTG > W3110S.gb/4208765‑4208900 | aTTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCActc > 1:1196568/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:872611/1‑71 (MQ=255) tGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGa > 1:966117/1‑71 (MQ=255) cccccAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATc > 1:1946295/1‑71 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCgg < 1:464851/71‑1 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGAt > 1:560593/1‑61 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:289264/71‑1 (MQ=255) cGGACGGGCCATCTGGTCCAGCGCCTCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCg > 1:2423516/1‑62 (MQ=255) gACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgt < 1:1442887/55‑1 (MQ=255) gACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAgt < 1:652923/71‑1 (MQ=255) cGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGa > 1:1054458/1‑55 (MQ=255) ggCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:2321401/64‑1 (MQ=255) tCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTtgtg < 1:1016875/47‑1 (MQ=255) tCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTa < 1:969397/54‑1 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:475675/1‑70 (MQ=255) gTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:2265023/55‑1 (MQ=255) gTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc < 1:1391599/68‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:1153467/71‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:543820/71‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:2444926/71‑1 (MQ=255) cAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTa < 1:1661609/70‑1 (MQ=255) gcgcCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc < 1:2797904/63‑1 (MQ=255) gccgcTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACtt > 1:1922077/1‑70 (MQ=255) cccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAcc > 1:449525/1‑70 (MQ=255) ccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCa < 1:2389376/71‑1 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCg < 1:2280521/37‑1 (MQ=255) tATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCaa > 1:499813/1‑62 (MQ=255) aTGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAg > 1:2085249/1‑38 (MQ=255) aTGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTg > 1:870215/1‑48 (MQ=255) tGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAg > 1:1823471/1‑37 (MQ=255) gCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAcc < 1:1426117/61‑1 (MQ=255) gCAAAACATTTCACTCTTGTGATCGCTAACCGGAGTGTCAATTTGCCATTAACTCCAAAcc < 1:574596/61‑1 (MQ=255) cAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAAtt > 1:2692951/1‑42 (MQ=255) cAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:1172840/71‑1 (MQ=255) cAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:1503266/71‑1 (MQ=255) cAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:2339788/71‑1 (MQ=255) aCATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTaa > 1:2630917/1‑47 (MQ=255) aCATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAc > 1:654793/1‑55 (MQ=255) cATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAc > 1:1927414/1‑54 (MQ=255) tttCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:1211470/64‑1 (MQ=255) tCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCt > 1:1806457/1‑70 (MQ=255) tCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCt > 1:2062590/1‑70 (MQ=255) cACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCt > 1:590155/1‑69 (MQ=255) cACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTg < 1:1224801/70‑1 (MQ=255) | ATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTG > W3110S.gb/4208765‑4208900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |