| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,225,730 | G→A | 11.8% | T117I (ACT→ATT) | arpA ← | regulator of acetyl CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,225,730 | 0 | G | A | 11.8% | 95.2 / 4.5 | 34 | T117I (ACT→ATT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (2/2); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGT > W3110S.gb/4225658‑4225798 | ccATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTA‑‑TGATTTTAGGTTTAATTCTTGTTTATCAagag < 1:1341943/71‑1 (MQ=255) tAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg > 1:44824/1‑71 (MQ=255) aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag > 1:2747605/1‑68 (MQ=255) aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg < 1:295684/70‑1 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:2582263/1‑71 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1276009/1‑71 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:2088409/1‑71 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1909988/1‑71 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1682121/1‑71 (MQ=255) aTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc < 1:1215939/70‑1 (MQ=255) cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt > 1:243740/1‑70 (MQ=255) cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt > 1:1342989/1‑70 (MQ=255) gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa < 1:423490/69‑1 (MQ=255) aTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa > 1:1575500/1‑70 (MQ=255) tctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGACGTaaaa > 1:2801069/1‑70 (MQ=255) ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtgt > 1:2808045/1‑51 (MQ=255) ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAtt > 1:695705/1‑71 (MQ=255) ttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa < 1:2732600/60‑1 (MQ=255) tATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:478793/61‑1 (MQ=255) tATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:1247919/61‑1 (MQ=255) tGATTTTAGGTTTAATTCTTGTTTATCAAGAgtgt > 1:987467/1‑35 (MQ=255) tGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtttt > 1:1958590/1‑70 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:485286/70‑1 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTACATTAGGTATTTTTCaaa < 1:2379593/70‑1 (MQ=255) tAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCAATTAGGTATTTTTCAAat < 1:1683575/70‑1 (MQ=255) gTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:153987/48‑1 (MQ=255) tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTATGTATTTTTCAAATATTgg < 1:836912/71‑1 (MQ=255) tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata < 1:2387043/67‑1 (MQ=255) tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTgg < 1:1516535/71‑1 (MQ=255) tAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTgggg > 1:1418655/1‑71 (MQ=255) aTTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGc < 1:908283/70‑1 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt < 1:2347073/43‑1 (MQ=255) ttGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg > 1:96934/1‑71 (MQ=255) gTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGt < 1:2580647/70‑1 (MQ=255) gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg < 1:829229/69‑1 (MQ=255) gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg < 1:558176/69‑1 (MQ=255) tttATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGt < 1:1437893/69‑1 (MQ=255) ttATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata > 1:1425020/1‑55 (MQ=255) tATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGttt > 1:673959/1‑69 (MQ=255) aaGAGTGTCTGAGCGTTGAGGTAAAATTTACATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:1495855/70‑1 (MQ=255) gagTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgt < 1:2321299/71‑1 (MQ=255) | CCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGT > W3110S.gb/4225658‑4225798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |