| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 712,019 | G→A | 79.0% | intergenic (‑132/+8) | fldA ← / ← ybfE | flavodoxin 1/LexA regulated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 712,019 | 0 | G | A | 79.0% | 74.5 / 17.7 | 38 | intergenic (‑132/+8) | fldA/ybfE | flavodoxin 1/LexA regulated protein |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base A (19/11); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAT > W3110S.gb/711952‑712083 | tCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATgg < 1:217279/71‑1 (MQ=255) cATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATgg > 1:505616/1‑70 (MQ=255) cATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATgg > 1:1167309/1‑70 (MQ=255) cATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATgg > 1:1529908/1‑70 (MQ=255) aCAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:2194163/71‑1 (MQ=255) aCAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:184600/71‑1 (MQ=255) aCAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAAc < 1:2028753/71‑1 (MQ=255) cAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAAc > 1:1294556/1‑70 (MQ=255) ttGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGa > 1:2117734/1‑71 (MQ=255) ttGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGa > 1:2390207/1‑71 (MQ=255) tCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAAc < 1:1115693/53‑1 (MQ=255) tCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGAc < 1:2257942/64‑1 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:1017879/1‑51 (MQ=255) aaCGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGGCTACGCAGcgccgc > 1:2554089/1‑71 (MQ=255) aaCGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccgc > 1:1354146/1‑71 (MQ=255) cGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAg > 1:962228/1‑63 (MQ=255) cGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccgc > 1:860957/1‑69 (MQ=255) gCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGtt < 1:2486520/71‑1 (MQ=255) gACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGc < 1:2189394/54‑1 (MQ=255) aCTGCACACTGTGCTACATGAAAATGGAAATTTaaa > 1:1782862/1‑36 (MQ=255) aCTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGc < 1:1096556/53‑1 (MQ=255) cTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1650150/1‑71 (MQ=255) tGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCgccgcc < 1:178729/60‑1 (MQ=255) tGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCgccgcc < 1:1327376/60‑1 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGa > 1:1165886/1‑35 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGcc > 1:371769/1‑39 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGa > 1:2441153/1‑43 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:1720196/1‑70 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTa < 1:1960265/71‑1 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTa < 1:2031369/71‑1 (MQ=255) acacTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTa < 1:2556103/70‑1 (MQ=255) acacTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTa < 1:1040670/70‑1 (MQ=255) acTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccgc < 1:1126199/54‑1 (MQ=255) gtgCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtct > 1:542720/1‑71 (MQ=255) cATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGa > 1:1759983/1‑71 (MQ=255) cATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGa > 1:1059947/1‑71 (MQ=255) gAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAt > 1:1436257/1‑69 (MQ=255) aaaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:89229/1‑52 (MQ=255) | TCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAT > W3110S.gb/711952‑712083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |