| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,245,658 | G→A | 47.6% | S63S (AGC→AGT) | xylE ← | D‑xylose transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,245,658 | 0 | G | A | 47.6% | ‑1.7 / 26.3 | 21 | S63S (AGC→AGT) | xylE | D‑xylose transporter |
| Reads supporting (aligned to +/- strand): ref base G (9/2); new base A (8/2); total (17/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
AATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAA > W3110S.gb/4245588‑4245722 | aaTCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgcg < 1:2036899/71‑1 (MQ=255) acgacgACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTgg > 1:2023503/1‑71 (MQ=255) tACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCTaa > 1:1423002/1‑70 (MQ=255) cTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaaa > 1:1458367/1‑61 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTgg > 1:2043683/1‑71 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTgg > 1:1826697/1‑71 (MQ=255) gAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCg > 1:1960042/1‑70 (MQ=255) ggCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt < 1:1242956/71‑1 (MQ=255) gCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt < 1:1445016/70‑1 (MQ=255) gccgATGATGCAACCAATCAGAGCACTGGCCACGCaaaa > 1:1449405/1‑39 (MQ=255) gccgATGATGCAACCAATCAGAGCACTGGCCACGCaaaa > 1:573880/1‑39 (MQ=255) gccgATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACtt > 1:1614832/1‑70 (MQ=255) gccgATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACtt > 1:1768338/1‑70 (MQ=255) gatgCAACCAATCAGAGCGCTGGCCACGCAAAACCCTaa > 1:1656036/1‑39 (MQ=255) atgCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAggg < 1:1684522/43‑1 (MQ=255) atgCAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAggg < 1:1494447/43‑1 (MQ=255) gCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtgtg > 1:1544704/1‑71 (MQ=255) gCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtgtg > 1:583280/1‑71 (MQ=255) aaTCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGAGGAGca > 1:2668785/1‑70 (MQ=255) agagCGCTGGCCACTCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaaca > 1:1161019/1‑69 (MQ=255) agagCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaacaa > 1:85890/1‑70 (MQ=255) agagCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaaca > 1:1730159/1‑69 (MQ=255) | AATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAA > W3110S.gb/4245588‑4245722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |