| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,354,061 | C→T | 15.9% | G218G (GGG→GGA) | dcuR ← | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,354,061 | 0 | C | T | 15.9% | 141.6 / 18.1 | 57 | G218G (GGG→GGA) | dcuR | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Reads supporting (aligned to +/- strand): ref base C (17/30); new base T (5/4); total (22/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACG > W3110S.gb/4353992‑4354129 | ccGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGcc > 1:2771826/1‑71 (MQ=255)ccGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGcc > 1:2421827/1‑71 (MQ=255) aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:1652211/70‑1 (MQ=255) atatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCAt < 1:238351/71‑1 (MQ=255) atatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:2503277/71‑1 (MQ=255) gTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATg < 1:761707/70‑1 (MQ=255) gTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATg < 1:1558020/70‑1 (MQ=255) tttCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGa < 1:289423/71‑1 (MQ=255) gtagtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTg < 1:839922/71‑1 (MQ=255) gtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGt < 1:1905108/70‑1 (MQ=255) tgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:369398/56‑1 (MQ=255) agtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCAGTGACGCAATaa > 1:738251/1‑56 (MQ=255) agtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAAc > 1:2103482/1‑68 (MQ=255) gtgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGa < 1:2395041/71‑1 (MQ=255) gtgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGa < 1:804038/71‑1 (MQ=255) gtgtGCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:885228/50‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:676722/50‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:2575132/50‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:2102348/50‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAt > 1:451261/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:169341/39‑1 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:80980/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:293699/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:1086643/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:1724819/1‑71 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTgg > 1:781246/1‑71 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTgg > 1:1249541/1‑71 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTgg > 1:2524289/1‑71 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTgg > 1:342312/1‑71 (MQ=255) gCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:357738/43‑1 (MQ=255) ccTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTTGCa < 1:1889358/70‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGa > 1:803910/1‑44 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACTCCATAATGGATACTGGTGAACAAGATGTGGc < 1:1242404/65‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGGAGTTGa < 1:6270/71‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGa > 1:2557207/1‑71 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGa > 1:792262/1‑71 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTCGCAGTTGa > 1:2609167/1‑71 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGAATACTGGTGAACAAGATGTGGCAGtt < 1:774122/69‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:2041018/37‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGa < 1:1659678/43‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACaa < 1:47560/56‑1 (MQ=255) cggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAt > 1:906578/1‑56 (MQ=255) cggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccag > 1:967804/1‑71 (MQ=255) ggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc < 1:1146722/71‑1 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACaa < 1:119863/51‑1 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAc > 1:2253671/1‑66 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAc > 1:2081345/1‑66 (MQ=255) gtaacggtaaACTGGACG‑CCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagcca < 1:681939/71‑1 (MQ=255) ggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGAt < 1:103642/70‑1 (MQ=255) gtaaACTGGACGTCCAGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGAt > 1:1020523/1‑69 (MQ=255) taaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATg < 1:221554/69‑1 (MQ=255) taaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAg < 1:1452415/71‑1 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAcca > 1:1918060/1‑59 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg < 1:683924/70‑1 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg < 1:687291/70‑1 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg < 1:137140/70‑1 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg < 1:1229285/70‑1 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGt < 1:449435/71‑1 (MQ=255) cGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACg > 1:534273/1‑71 (MQ=255) | CCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACG > W3110S.gb/4353992‑4354129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |