| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,495,142 | C→T | 6.3% | G227E (GGA→GAA) | idnR ← | DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,495,142 | 0 | C | T | 6.3% | 188.8 / 3.1 | 64 | G227E (GGA→GAA) | idnR | DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
| Reads supporting (aligned to +/- strand): ref base C (25/33); new base T (2/2); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
TCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATC > W3110S.gb/4495073‑4495212 | tCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc > 1:1048729/1‑71 (MQ=255)tCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc > 1:1064456/1‑71 (MQ=255)tCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc > 1:175836/1‑71 (MQ=255) gTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCTTTTCTa < 1:2389/71‑1 (MQ=255) gTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTGCTa < 1:719068/71‑1 (MQ=255) aTTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTACTaaa > 1:1891061/1‑70 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTTCTAaatg < 1:1813862/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:1706049/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:1452128/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:2090353/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:1300377/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:2549646/71‑1 (MQ=255) ttGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:1088126/71‑1 (MQ=255) tGCAAAATACACCGTCTAAATCCGGGTTTTCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatgaatg < 1:2395982/71‑1 (MQ=255) tGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatgaatg < 1:1713235/71‑1 (MQ=255) cAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATgg < 1:1952166/70‑1 (MQ=255) cAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATgg < 1:507878/70‑1 (MQ=255) cAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGa > 1:2331698/1‑71 (MQ=255) aaTACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATga < 1:2722614/71‑1 (MQ=255) aaTACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATga < 1:2802265/71‑1 (MQ=255) aaTACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATga < 1:544654/71‑1 (MQ=255) aaTACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATga < 1:9829/71‑1 (MQ=255) aaTACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATga < 1:1555219/71‑1 (MQ=255) cacCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGAt > 1:2502545/1‑70 (MQ=255) cacCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATg > 1:2034943/1‑71 (MQ=255) cacCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATg > 1:2012441/1‑71 (MQ=255) cacCGTCTAAATCCGGGTTTGCGATAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATg > 1:437787/1‑71 (MQ=255) cGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGgcg > 1:1089280/1‑71 (MQ=255) tAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATgaga > 1:650540/1‑62 (MQ=255) tCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGAtt < 1:2336399/71‑1 (MQ=255) gTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg < 1:1681018/42‑1 (MQ=255) tGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGa > 1:592452/1‑45 (MQ=255) tGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGt < 1:1909323/70‑1 (MQ=255) tGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGt > 1:26557/1‑70 (MQ=255) tGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGt < 1:1446362/70‑1 (MQ=255) tGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGt < 1:596899/70‑1 (MQ=255) cgcTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAAt > 1:461603/1‑71 (MQ=255) aGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATAGCGCGTGGATTCATGCGTAATGGGGa < 1:1894752/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:1267835/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:1124753/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:1500807/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:1336441/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:783825/71‑1 (MQ=255) aTCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt > 1:135092/1‑71 (MQ=255) tCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGAtt < 1:2176827/50‑1 (MQ=255) tCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGAtt < 1:556452/50‑1 (MQ=255) aCGCATTAACTGCATTTCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTAt > 1:280392/1‑71 (MQ=255) aCGCATTAACTGCATTCCTAAATGAATGGATGAGATgg > 1:2252664/1‑38 (MQ=255) aCGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTAt > 1:1241023/1‑71 (MQ=255) gCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAAt > 1:1035130/1‑56 (MQ=255) ttAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATgcagc < 1:2315135/71‑1 (MQ=255) aaCTGCATTTCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATgg > 1:2128933/1‑53 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATg > 1:2534557/1‑52 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:1300829/1‑71 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:1796503/1‑71 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:2001290/1‑71 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:108552/1‑71 (MQ=255) aaCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:855947/1‑71 (MQ=255) aCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTAt > 1:1722207/1‑63 (MQ=255) aCTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTAt > 1:2583241/1‑63 (MQ=255) cATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCga < 1:355169/43‑2 (MQ=255) cATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcat > 1:1327064/1‑66 (MQ=255) ttCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAg < 1:2310831/52‑1 (MQ=255) ttCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:308720/54‑1 (MQ=255) ttCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGAt < 1:1055124/54‑1 (MQ=255) tCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTaa > 1:1577567/1‑42 (MQ=255) tCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTa > 1:1475239/1‑55 (MQ=255) ccTAATTGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGcatcat > 1:1348895/1‑65 (MQ=255) ccTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATg > 1:1365431/1‑43 (MQ=255) ccTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGa > 1:2551135/1‑51 (MQ=255) ccTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATcatcgcatc > 1:481422/1‑71 (MQ=255) ccTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATcatcgcatc > 1:2353629/1‑71 (MQ=255) | TCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATC > W3110S.gb/4495073‑4495212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |