| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 767,363 | T→C | 62.5% | L320L (TTG→CTG) | mngA → | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 767,363 | 0 | T | C | 62.5% | 17.8 / 21.9 | 24 | L320L (TTG→CTG) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base C (5/10); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
AACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTAC > W3110S.gb/767295‑767405 | aaCCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTg < 1:1501607/71‑1 (MQ=255) cGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGAtt < 1:2035003/71‑1 (MQ=255) ggTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTg > 1:985967/1‑71 (MQ=255) aaaaGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGAt > 1:1363601/1‑65 (MQ=255) aaaaGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgc < 1:782162/71‑1 (MQ=255) aGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCggg < 1:1641409/71‑1 (MQ=255) aGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCggg > 1:923279/1‑71 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGt > 1:1837207/1‑59 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTc > 1:1906829/1‑60 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGAt > 1:2025900/1‑56 (MQ=255) cTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTg < 1:637980/45‑1 (MQ=255) cAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTcgc < 1:1348193/71‑1 (MQ=255) ggCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:1730132/71‑1 (MQ=255) ggCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:29515/71‑1 (MQ=255) aCTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTAt < 1:1102904/71‑1 (MQ=255) cTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTAt < 1:2043181/70‑1 (MQ=255) cTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTAt < 1:2046514/70‑1 (MQ=255) tGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCAc < 1:992304/49‑1 (MQ=255) gTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTAc > 1:568929/1‑71 (MQ=255) gTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTAc > 1:137813/1‑71 (MQ=255) ttGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTcgcg < 1:594547/39‑1 (MQ=255) ttGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTcgcg < 1:1813353/39‑1 (MQ=255) tctcTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:553746/54‑1 (MQ=255) tctcTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:2354135/54‑1 (MQ=255) | AACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTAC > W3110S.gb/767295‑767405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |