| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,549,754 | G→A | 80.8% | intergenic (+43/‑24) | fimC → / → fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,549,754 | 0 | G | A | 80.8% | 53.1 / 8.7 | 26 | intergenic (+43/‑24) | fimC/fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (11/10); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
AAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACA > W3110S.gb/4549688‑4549814 | aaaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGt > 1:1939447/1‑70 (MQ=255) gggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTCAATAAAAAGAATTGACTGCCAGGTGATTTTaa < 1:2360584/70‑1 (MQ=255) taaTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGaat < 1:1878328/70‑1 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:1845110/71‑1 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:2737510/71‑1 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:2698542/71‑1 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGt > 1:2352574/1‑71 (MQ=255) aaTTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTa < 1:1102103/44‑1 (MQ=255) tttttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAg > 1:392182/1‑44 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGaa > 1:2341513/1‑51 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt > 1:986620/1‑70 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt < 1:411987/70‑1 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt > 1:1128879/1‑70 (MQ=255) gCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc < 1:2565297/71‑1 (MQ=255) aaTAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:2303952/1‑71 (MQ=255) aaTAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:2300609/1‑71 (MQ=255) tAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTaa < 1:470439/61‑1 (MQ=255) tAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:865722/1‑69 (MQ=255) aaaaGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCatat > 1:2355627/1‑49 (MQ=255) gAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg > 1:2002291/1‑66 (MQ=255) aTTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCatat > 1:1644060/1‑43 (MQ=255) aTTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGATTTTAAGACTTTACCAGCGAAac > 1:1976249/1‑70 (MQ=255) aTTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTa < 1:82670/60‑1 (MQ=255) aTTGACTGCCAGGTGATTTTAAGCCGGAGCAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca < 1:2037733/71‑1 (MQ=255) ttGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt < 1:278195/50‑1 (MQ=255) ttGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTaa < 1:1357755/52‑1 (MQ=255) | AAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACA > W3110S.gb/4549688‑4549814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |