| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,558,086 | G→A | 58.1% | A168A (GCG→GCA) | uxuB → | D‑mannonate oxidoreductase, NAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,558,086 | 0 | G | A | 58.1% | 9.9 / 36.4 | 31 | A168A (GCG→GCA) | uxuB | D‑mannonate oxidoreductase, NAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (8/5); new base A (10/8); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
CTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTG > W3110S.gb/4558021‑4558150 | cTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcg < 1:2615037/71‑1 (MQ=255) aTCAAGAACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTtgcgtct > 1:135887/1‑71 (MQ=255) aaGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtctgcg > 1:1444911/1‑71 (MQ=255) gcgcCGAAGTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGtt > 1:2630356/1‑71 (MQ=255) cgcCGAAGTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGtt < 1:2410529/70‑1 (MQ=255) ccGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGa < 1:837178/61‑1 (MQ=255) aaGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGaaaa < 1:1266921/50‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt < 1:1236235/69‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt < 1:670400/69‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:1272108/1‑71 (MQ=255) tCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt < 1:2563088/68‑1 (MQ=255) tCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa < 1:2647429/70‑1 (MQ=255) cgcgATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGtt > 1:1303352/1‑60 (MQ=255) cgcgATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGt > 1:94396/1‑71 (MQ=255) cgcgATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGt < 1:2242476/71‑1 (MQ=255) cgcgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATg > 1:2174658/1‑70 (MQ=255) cgcgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATg > 1:1684861/1‑70 (MQ=255) cgcgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGt > 1:2744832/1‑71 (MQ=255) aTTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg < 1:1295503/71‑1 (MQ=255) ggTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACgg > 1:668230/1‑58 (MQ=255) ggTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:2390725/1‑61 (MQ=255) aCATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:2401715/1‑57 (MQ=255) aCATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:1372143/1‑57 (MQ=255) aCATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATaa < 1:2622213/70‑1 (MQ=255) tcgtcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa < 1:1709041/54‑1 (MQ=255) tcgtcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa < 1:1537723/54‑1 (MQ=255) gtcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgtgcg > 1:238852/1‑71 (MQ=255) gtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGt > 1:2242090/1‑43 (MQ=255) gtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGt > 1:1005276/1‑43 (MQ=255) gtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:1484506/1‑52 (MQ=255) gAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgtgcgtg > 1:1301756/1‑70 (MQ=255) | CTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTG > W3110S.gb/4558021‑4558150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |