Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	100.4 / NA	35	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/20);  total (15/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACG  >  W3110S.gb/4563529‑4563651                                                             |                                                               agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc                                                      <  1:537175/71‑1 (MQ=255)    agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTCTAACGCCATTTTACTGTCGcc                                                   <  1:2147947/71‑1 (MQ=255)     gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc                                                  <  1:1883965/71‑1 (MQ=255)      cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                         <  1:2793289/63‑1 (MQ=255)      cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                         <  1:1578212/63‑1 (MQ=255)                ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                       <  1:1817385/71‑1 (MQ=255)                   acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                         <  1:1735470/50‑1 (MQ=255)                   acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:103691/71‑1 (MQ=255)                   acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:684458/71‑1 (MQ=255)                   acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:822936/71‑1 (MQ=255)                      tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:1231306/68‑1 (MQ=255)                      tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:190271/68‑1 (MQ=255)                      tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                    <  1:94338/68‑1 (MQ=255)                         cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                              <  1:587157/71‑1 (MQ=255)                            ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                       <  1:475701/59‑1 (MQ=255)                              ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                     >  1:2033890/1‑59 (MQ=255)                              ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                         >  1:208336/1‑71 (MQ=255)                               tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                         <  1:2789039/70‑1 (MQ=255)                                        tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg               >  1:1618981/1‑71 (MQ=255)                                         cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                  >  1:17290/1‑51 (MQ=255)                                         cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                            >  1:1325857/1‑57 (MQ=255)                                         cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg               >  1:826285/1‑70 (MQ=255)                                         cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg               >  1:560056/1‑70 (MQ=255)                                          aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                          >  1:551437/1‑42 (MQ=255)                                          aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc              >  1:259554/1‑70 (MQ=255)                                          aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc              >  1:2139933/1‑70 (MQ=255)                                             aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                      <  1:551655/59‑1 (MQ=255)                                             aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                      <  1:1539381/59‑1 (MQ=255)                                             aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                      <  1:973444/59‑1 (MQ=255)                                             aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa           >  1:697063/1‑70 (MQ=255)                                             aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc          >  1:2692819/1‑71 (MQ=255)                                              aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc         <  1:1553973/71‑1 (MQ=255)                                                     gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcg  >  1:397913/1‑71 (MQ=255)                                                      tAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                     >  1:391409/1‑35 (MQ=255)                                                          gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa                       >  1:2128209/1‑45 (MQ=255)                                                             |                                                               AGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACG  >  W3110S.gb/4563529‑4563651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑