| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,591,162 | C→A | 75.0% | V112V (GTG→GTT) | hsdR ← | endonuclease R |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,591,162 | 0 | C | A | 75.0% | 29.6 / 24.4 | 32 | V112V (GTG→GTT) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base C (6/2); new base A (14/10); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGG > W3110S.gb/4591099‑4591226 | aGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCt > 1:1103907/1‑71 (MQ=255) gCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTg < 1:1539937/71‑1 (MQ=255) gCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTg < 1:2398713/71‑1 (MQ=255) cAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGt < 1:2454529/71‑1 (MQ=255) cGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc < 1:2610648/71‑1 (MQ=255) ggAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc > 1:948328/1‑70 (MQ=255) ggAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc > 1:2361493/1‑70 (MQ=255) gAACCCGAGTCTCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCaa < 1:951857/71‑1 (MQ=255) gTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGt < 1:2017410/71‑1 (MQ=255) gCAGGCACATCTGGGCATCGTTGAGATCGTTATGATTTTCGTGAACCGCCTGGTTACCAATGCGGCGTAAt > 1:2479294/1‑71 (MQ=255) gCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTaa > 1:1744808/1‑70 (MQ=255) cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTtgt > 1:1943741/1‑70 (MQ=255) acaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCaa > 1:201384/1‑54 (MQ=255) aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa < 1:33268/71‑1 (MQ=255) tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGa < 1:1149815/68‑1 (MQ=255) tGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCaa > 1:2551243/1‑49 (MQ=255) tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc < 1:1762684/41‑1 (MQ=255) tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc < 1:2457444/41‑1 (MQ=255) gTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:122314/1‑71 (MQ=255) gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa > 1:888893/1‑62 (MQ=255) gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:1970420/1‑71 (MQ=255) gagaTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgt > 1:2163616/1‑71 (MQ=255) tCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtc < 1:904704/71‑1 (MQ=255) cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTaa > 1:2751523/1‑43 (MQ=255) cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa > 1:1724685/1‑54 (MQ=255) cGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtca > 1:2503049/1‑71 (MQ=255) cGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtca > 1:2259708/1‑71 (MQ=255) cGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtca > 1:2691632/1‑71 (MQ=255) atatTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATcaaca < 1:266145/70‑1 (MQ=255) atTCGTGAACCGCCTGGTTACCAATGCGGCGTAAt > 1:1787631/1‑35 (MQ=255) cGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa > 1:2646505/1‑42 (MQ=255) cGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAgg > 1:2683221/1‑69 (MQ=255) | AGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGG > W3110S.gb/4591099‑4591226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |