| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,616,051 | T→C | 11.3% | intergenic (+366/‑27) | prfC → / → osmY | peptide chain release factor RF‑3/periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,616,051 | 0 | T | C | 11.3% | 158.0 / 11.8 | 62 | intergenic (+366/‑27) | prfC/osmY | peptide chain release factor RF‑3/periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (33/22); new base C (3/4); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTG > W3110S.gb/4615982‑4616120 | cATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTa > 1:915776/1‑71 (MQ=255)cATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTa > 1:666464/1‑71 (MQ=255) aTTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTAc < 1:1989786/71‑1 (MQ=255) gACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg > 1:1931167/1‑71 (MQ=255) gACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg > 1:2456289/1‑71 (MQ=255) gACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg > 1:145124/1‑71 (MQ=255) agagGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa < 1:41934/70‑1 (MQ=255) gagGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:448975/1‑69 (MQ=255) gggTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAgg < 1:79971/71‑1 (MQ=255) gggTGAAGTCTATCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAgg < 1:1171314/71‑1 (MQ=255) gTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg < 1:1758000/57‑1 (MQ=255) gTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg < 1:1145581/57‑1 (MQ=255) gAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACg > 1:1257643/1‑53 (MQ=255) gAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAAAATACAGGAAt > 1:704879/1‑70 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:378206/1‑55 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:766786/1‑55 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:685808/1‑55 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACa > 1:2425285/1‑57 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc < 1:353126/71‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:495015/68‑1 (MQ=255) cTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc > 1:1166524/1‑70 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa < 1:2476180/49‑1 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATg < 1:2166764/71‑1 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:2606981/70‑1 (MQ=255) ttCAGCAATCCAACCTCGTTTTGCCAGGCTCAAATTACGAGCAAACATa < 1:251615/49‑1 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTAc > 1:1479217/1‑36 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa < 1:2734630/70‑1 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGa > 1:108291/1‑61 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGa > 1:109154/1‑64 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGAc < 1:2218755/70‑1 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGAc < 1:122161/70‑1 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATc < 1:937362/55‑1 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:454258/1‑71 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:1868757/1‑71 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:1510558/1‑71 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:2610975/1‑71 (MQ=255) aTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATc < 1:2242762/54‑1 (MQ=255) gCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACt > 1:1432282/1‑70 (MQ=255) gCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACt > 1:1357232/1‑70 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:2108693/1‑62 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:1832538/1‑70 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:1189906/1‑70 (MQ=255) cAACCTCGTGTTGCCAGGCTAAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:1741922/1‑70 (MQ=255) aCCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAg < 1:1245842/71‑1 (MQ=255) aCCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAg < 1:1223861/71‑1 (MQ=255) aCCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGAc < 1:294601/61‑1 (MQ=255) ccTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:2515518/1‑36 (MQ=255) ccTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:1570349/1‑36 (MQ=255) ccTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:886774/1‑36 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGAtt > 1:946166/1‑71 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:295580/55‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCTaa < 1:2792246/71‑1 (MQ=255) ttGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGAc < 1:386950/47‑1 (MQ=255) ttGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGa > 1:913991/1‑70 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:2126697/1‑55 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaaaa > 1:1615552/1‑71 (MQ=255) ccAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAAc < 1:1391285/71‑1 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:2721279/1‑43 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:330661/1‑71 (MQ=255) cAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgctg > 1:2393398/1‑71 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgctg < 1:2046175/70‑1 (MQ=255) aaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAActct < 1:2164125/65‑1 (MQ=255) ttACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:93254/41‑1 (MQ=255) ttACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTgctggctg > 1:2363070/1‑71 (MQ=255) | CATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTG > W3110S.gb/4615982‑4616120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |