| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 847,104 | A→G | 86.2% | R146R (CGT→CGC) | glnP ← | glutamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 847,104 | 0 | A | G | 86.2% | 75.2 / 4.6 | 29 | R146R (CGT→CGC) | glnP | glutamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (13/12); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGC > W3110S.gb/847041‑847166 | cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgacg > 1:122009/1‑69 (MQ=255)cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgacg > 1:1769838/1‑69 (MQ=255) tAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGc > 1:2235724/1‑70 (MQ=255) cAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCg > 1:1034483/1‑71 (MQ=255) cGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTa < 1:2120352/71‑1 (MQ=255) gAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTa < 1:2138816/70‑1 (MQ=255) tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATg < 1:1255372/71‑1 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGc < 1:2783503/51‑1 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGc < 1:2486113/51‑1 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAg > 1:1587160/1‑53 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAg > 1:1319598/1‑53 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGAATGCGACGc < 1:1807214/51‑1 (MQ=39) aTGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCg < 1:1536548/71‑1 (MQ=255) aTGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCg < 1:614651/71‑1 (MQ=255) cTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCg > 1:1019705/1‑52 (MQ=255) cTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAAt > 1:1414950/1‑71 (MQ=255) ttACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCg < 1:2357927/53‑1 (MQ=255) tACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa > 1:155156/1‑70 (MQ=255) aCCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGtt > 1:536878/1‑58 (MQ=255) aCCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa > 1:189464/1‑69 (MQ=255) ccAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGa < 1:1809617/71‑1 (MQ=255) aGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc > 1:202019/1‑71 (MQ=255) aGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAc > 1:300329/1‑70 (MQ=255) ggcggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGaaa > 1:1920769/1‑64 (MQ=255) ggcggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGaaa > 1:1730070/1‑64 (MQ=255) gcggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg < 1:1458252/47‑1 (MQ=255) cggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCaa < 1:2284328/55‑1 (MQ=255) cggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCaa < 1:628648/55‑1 (MQ=255) ggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATgg > 1:2275499/1‑46 (MQ=255) ggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGaa > 1:5517/1‑60 (MQ=255) ggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgcgc < 1:1155034/71‑1 (MQ=255) | CACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGC > W3110S.gb/847041‑847166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |