| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 884,617 | G→A | 79.0% | V175I (GTT→ATT) | cmr → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 884,617 | 0 | G | A | 79.0% | 34.0 / 6.0 | 19 | V175I (GTT→ATT) | cmr | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (5/10); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCAC > W3110S.gb/884551‑884684 | gCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATgtttattt < 1:1223645/70‑1 (MQ=255)gCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATgtttattt < 1:1879714/70‑1 (MQ=255) cTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGtttgttttg < 1:1533107/71‑1 (MQ=255) cTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGtttgttttg < 1:1644763/71‑1 (MQ=255) tGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCAt > 1:2409647/1‑71 (MQ=255) cTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGc < 1:1918370/71‑1 (MQ=255) tggGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGc < 1:329766/58‑1 (MQ=255) cgcgGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGTATTGGCAGCGATCTCCt < 1:2375316/71‑1 (MQ=255) cggcgTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCttt < 1:1176932/71‑1 (MQ=255) gTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCg < 1:289903/71‑1 (MQ=255) aTCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCttttt < 1:1084540/65‑1 (MQ=255) aTCCATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCttttt < 1:2001339/65‑1 (MQ=255) tCCATGTGCTGCCCTGGGAGGGGATGTttattttgtt > 1:1873199/1‑37 (MQ=255) ccATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTg > 1:520416/1‑70 (MQ=255) cATGTGCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGc < 1:805747/70‑1 (MQ=255) tgtgCTGCCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCg < 1:943989/49‑1 (MQ=255) tgctgcCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGAtct < 1:1916343/51‑1 (MQ=255) tgctgcCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAAc > 1:2730834/1‑69 (MQ=255) tgctgcCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACgt > 1:151582/1‑70 (MQ=255) tgctgcCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAAc > 1:2774711/1‑69 (MQ=255) tgctgcCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAAc > 1:2800758/1‑69 (MQ=255) ctgcCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGc < 1:2109996/71‑1 (MQ=255) ctgcCCTGGGAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGc < 1:134378/71‑1 (MQ=255) gggAGGGGATGTTTATTTTGTTTGCCGCATTGGCAGCGATCTCCttttt > 1:2111083/1‑49 (MQ=255) tgtttattTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGc < 1:2511457/48‑1 (MQ=255) tgtttattTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGc < 1:1442847/48‑1 (MQ=255) tgtttattTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGc < 1:1410462/70‑1 (MQ=255) tttgttttgtTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGcc > 1:2197579/1‑59 (MQ=255) tttattttgtTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCAc > 1:2769825/1‑71 (MQ=255) tgttttgtttGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCaa > 1:128119/1‑46 (MQ=255) | GCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCAC > W3110S.gb/884551‑884684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |